Physical Aspects of the Spin Labelling Technique

  • Philippe F. Devaux
  • Jean Davoust
Conference paper
Part of the Nato Advanced Study Institutes Series book series (ASIC, volume 52)

Abstract

A brief review of the physical aspects of the spin labeling technique is given. Emphasis is put on the qualitative description of the phenomena which enable us to obtain information on molecular motion in biological and related systems. The conventional use of nitroxides, saturation transfer spectroscopy and electron spin-electron spin interactions are the three topics discussed.

Keywords

Microwave Anisotropy Hyde Nitroxides Aniso 

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References

  1. (1).
    Spin-labeling, theory and applications. Acad. Press, Ed. L. J. Berliner, Vol. I, 1976, Vol.11 in the press 1979.Google Scholar
  2. (2).
    A pure spin-label crystal would give rise to a single very broad line due to spin-spin interactions, as indicated in section IV.Google Scholar
  3. (3).
    In figure 1-c, the distribution function for the 3 lines is represented very crudely by 3 rectangles. The actual distribution is more complicated but this figure at least shows that the 3 nuclear spins play unequivalent roles. This disymmetry is due to the g value.Google Scholar
  4. (4).
    The exact critical value is 54°7, this is because the distribution functions are not simple rectangles as indicated in figure 3.Google Scholar
  5. (5).
    Thomas D., Dalton L.R. and Hyde J.S. (1976). J. Chem. Phys., 65, 3006–3024.CrossRefGoogle Scholar
  6. (6).
    The actual power on the sample is one of the crucial parameter to determine; it requires a good calibration of the system.Google Scholar
  7. (7).
    Devaux P., Scandella C.J. and Mc Connell H.M. (1973), J. Magn.Res., 9, 474–485.Google Scholar
  8. (8).
    Favre E., Baroin A., Bienvenue A. and Devaux P.F. (1979), Biochem., 18, 1156–1162.CrossRefGoogle Scholar
  9. (9).
    Bienvenue A., Hervé P. and Devaux P.F. (1978), C.R. Acad. Sci. Paris, 287D, 247–1250.Google Scholar

Copyright information

© D. Reidel Publishing Company, Dordrecht, Holland 1980

Authors and Affiliations

  • Philippe F. Devaux
    • 1
  • Jean Davoust
    • 1
  1. 1.Institut de Biologie Physico-ChimiqueParisFrance

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