Cryoenzymology in Reverse Micelles

  • Balny Claude
  • Hui Bon Hoa Gaston
  • Douzou Pierre
Part of the The Jerusalem Symposia on Quantum Chemistry and Biochemistry book series (JSQC, volume 12)


Solutions of homogeneous and optically transparent micromicelles in nonpolar solvents of low viscosity, are used to investigate enzyme systems in pure aqueous media at subzero temperatures. The properties of such water microspheres are presented and their behavior studied as a function of their chemical composition, in a large temperature range Enzymatic reactions are described. Rapid mixing of very low viscosity micromicellar solutions to record fast kinetic are discussed in comparison with results obtained in bulk water or in mixed hydro–organic solvents.


Reverse Micelle Bulk Water Micellar Solution Nonpolar Solvent Water Pool 
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Copyright information

© D. Reidel Publishing Company 1979

Authors and Affiliations

  • Balny Claude
    • 1
    • 2
  • Hui Bon Hoa Gaston
    • 1
    • 2
  • Douzou Pierre
    • 1
    • 2
  1. 1.U-128 INSERMMontpellier cédexFrance
  2. 2.Institut de Biologie Physico-ChimiqueParisFrance

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