Fast Kinetics by NMR: Binding and Release of Cations by Biomolecules
Because of its high sensitivity, sodium-23 nuclear magnetic resonance (NMR) is useful for the study of ion-substrate interactions (1). Complex formation induces a relaxation enhancement of the nuclear spins manifested by line broadening whereas the position of the peak is little affected. Under some conditions, such relaxation measurements can be used to determine the forward and the reverse rate constants for complex formation. In this paper we present examples of the determination of kinetic parameters by NMR.
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- (2).T.E. Bull, J. Magn. Res., 8, 341 (1972).Google Scholar
- (5a).J. Grandjean, P. Laszlo in “Protons and Ions involved in Fast Dynamics Phenomena”, P. Laszlo, Ed., Elsevier, Amsterdam, p. 373 (1978).Google Scholar
- (6).A. Cornélls, P. Laszlo, Biochem, in press.Google Scholar