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Computer-Controlled Repetitive Operation of a Stopped-Flow Spectrophotometer with a Magnetic Valve in Place of the Stopping Syringe

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Techniques and Applications of Fast Reactions in Solution

Part of the book series: NATO Advanced Study Institutes Series ((ASIC,volume 50))

Abstract

The stopped-flow technique (1,2) is the most flexible and widely used method for the study of rapid biochemical reactions. A major limitation, however, is the collection and analysis of the results. The introduction of laboratory on-line computers allowed a considerable increase in the accuracy and convenience of data collection and processing (3). A logical further advance is to allow the computer to control the operations of the apparatus. The major obstacle to this is the stopping syringe. We describe here how this may be replaced by a suitable electromagnetic valve, allowing the whole measuring cycle to be controlled by the computer.

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References

  1. Gibson, Q.H. (1969) Methods Enzymol. 16, 187–228

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  2. Chance, B. (197U) in Techniques of Chemistry, vol. 6 (Hammes, G.G. ed.) Part 2, pp. 5–62, Wiley-Interscience, New York.

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  3. DeSa, R.J. and Gibson, Q.H. (1969) Comput. Biomed. Res. 6, 216–227

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  4. Gibson, Q.H. and Milnes, L. (1964) Biochem. J. 91, 161-171

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© 1979 D. Reidel Publishing Company

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Cox, R.P., Ormicki, A., Degn, H. (1979). Computer-Controlled Repetitive Operation of a Stopped-Flow Spectrophotometer with a Magnetic Valve in Place of the Stopping Syringe. In: Gettins, W.J., Wyn-Jones, E. (eds) Techniques and Applications of Fast Reactions in Solution. NATO Advanced Study Institutes Series, vol 50. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-9490-4_3

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  • DOI: https://doi.org/10.1007/978-94-009-9490-4_3

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-009-9492-8

  • Online ISBN: 978-94-009-9490-4

  • eBook Packages: Springer Book Archive

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