Vital Staining of the Lysosomes in Normal Keratocytes and in Keratocytes from Macular Dystrophy

Abstract

It has been established that many basic or neutral substances can be fixed on the lysosomes of living cells (Koenig, 1963a and b; Allison et al., 1964a; Robbins et al., 1964; Allison et al., 1965a and b; Bastos et al., 1966; Zelenin, 1966; Barrett et al, 1968; Allison et al., 1973; Dingle et al., 1973b). Among these substances, there are fluorescent dyes, anti-inflammatory agents, cancerogenic products (Allison et al., 1964a) and some highly positively charged metals (Allison et al, 1966; Goldfischer, 1967; Allison et al., 1973)(Fig. 8).