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The Effect of Base Modification on Fidelity in Transcription

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Carcinogenesis: Fundamental Mechanisms and Environmental Effects

Part of the book series: The Jerusalem Symposia on Quantum Chemistry and Biochemistry ((JSQC,volume 13))

Abstract

Ribopolynucleotides containing a small number of various modified nucleotides were examined for fidelity in transcription using DNA- dependent RNA polymerase in the presence of Mn2+. When normal hydrogen bonding is blocked by a substituent, total ambiguity results with transcription continuing beyond the modified nucleotide. Examples are 3-MeC, 3-MeU, 1-MeA and O4-MeU. In the case of modifications of the exocyclic amino groups, in addition to the possible tautomeric shift with C, the orientation of the substituent plays a major role in determining fidelity. Thus N4-methoxy C acts only as U; N4-hydroxy C, N4-MeC, and N2-MeG act ambiguously; and N4-acetyl C base-pairs only with G. Three different substituents on the N6 of A did not affect A·U pairing, indicating an anti conformation.

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References

  1. Singer, B. and Kröger, M.: 1979 Prog, in Nucleic Acids Res. and Mol. Biol. 23, pp. 151–194

    Article  CAS  Google Scholar 

  2. Singer, B.: 1976 Nature (London) 264, pp. 333–339

    Article  CAS  Google Scholar 

  3. Singer, B., Bodell, w. J., Cleaver, J. E., Thomas, G. H., Rajewsky, M. G. and Thon, W.: 1978 Nature (London) 276, pp. 85–88.

    Article  CAS  Google Scholar 

  4. Kröger, M. and Singer, B.: 1979, Biochemistry 18, pp. 3493–3500

    Article  Google Scholar 

  5. Shearman, C. W. and Loeb, L. A.: 1977 Nature (London) 270, pp. 537–538

    Article  CAS  Google Scholar 

  6. Singer, B., Fraenkel-Conrat, H. and Kusśmierek, J. T.: 1970 Proc. Natl Acad. Sci (USA) 75, pp. 1722–1726

    Article  Google Scholar 

  7. Singer, B., Pergolizzi, R. G. and Grunberger, D.: 1979 Nucleic Acids Res. 6, pp. 1709–1719

    Article  CAS  Google Scholar 

  8. Singer, B.: 1979 J. Natl. Cancer Inst. 62, pp. 1329–1339.

    CAS  Google Scholar 

  9. Gerchman, L. L. and Ludlum, D. B.: 1973 Biochim. Biophys. Acta 300, pp. 310–316

    Google Scholar 

  10. Birnbaum, G. I., Kulikowski, T. and Shugar, D.: 1979 Can. J. Biochem 57, pp. 308–313

    Article  CAS  Google Scholar 

  11. Parthasarathy, R., Ginell, S. L., De, N. C., and Chheda, G. B. 1978 Biochem. Biophys. Res. Comm. 83, pp. 657–663

    Article  CAS  Google Scholar 

  12. Shoup, R. R., Miles, H. T., and Becker, E. D.: 1972 J. Phys. Chew. 76, pp. 64–70

    Article  CAS  Google Scholar 

  13. Engel, J. D. and von Hippel, P. H.: 1974 Biochemistry 13, pp. 4143–4158

    Article  CAS  Google Scholar 

  14. Becker, E. D., Miles, H. T. and Bradley, R. B.: 1965 J. Aroer. Chem. Soc. 87, pp. 5575–5582

    Article  CAS  Google Scholar 

  15. Brimacombe, R. L. C. and Reese, C. B.: 1966 J. Mol. Biol. 18, pp. 529–540

    Article  CAS  Google Scholar 

  16. Brown, D. M., Hewlins, M. J. E. and Schell, P.: 1968 J. Chem. Soc. (C), pp. 1925–1929

    Google Scholar 

  17. Budowsky, E. I., Sverdlov, E. D., and Spasokukotskaya, T. N.: 1972 Biochin. Biophys. Acta 287, pp. 195–210

    CAS  Google Scholar 

  18. Müller, W., Weber, II., Meyer, F. and Weissmann, C.: 1978 J. Mol. Biol. 124, pp. 343–358

    Article  Google Scholar 

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Author information

Authors and Affiliations

  1. Department of Molecular Biology and Virus Laboratory, University of California, Berkeley, California, 94720, USA

    B. Singer

Authors
  1. B. Singer
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Editor information

Editors and Affiliations

  1. Institut de Biologie Physico-Chimique (Fondation Edmond de Rothschild), Université Pierre et Marie Curie (Paris VI), Paris, France

    Bernard Pullman

  2. The Johns Hopkins University, Baltimore, Maryland, USA

    Paul O. P. Ts’o

  3. National Cancer Institute, Bethesda, Maryland, USA

    Harry Gelboin

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© 1980 D. Reidel Publishing Company

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Singer, B. (1980). The Effect of Base Modification on Fidelity in Transcription. In: Pullman, B., Ts’o, P.O.P., Gelboin, H. (eds) Carcinogenesis: Fundamental Mechanisms and Environmental Effects. The Jerusalem Symposia on Quantum Chemistry and Biochemistry, vol 13. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-9104-0_8

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  • DOI: https://doi.org/10.1007/978-94-009-9104-0_8

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-009-9106-4

  • Online ISBN: 978-94-009-9104-0

  • eBook Packages: Springer Book Archive

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