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DNA Repair in Human Cells Exposed to Combinations of Carcinogenic Agents

  • Conference paper
Book cover Carcinogenesis: Fundamental Mechanisms and Environmental Effects

Part of the book series: The Jerusalem Symposia on Quantum Chemistry and Biochemistry ((JSQC,volume 13))

Abstract

Normal human and XP2 fibroblasts were treated with UV plus UV-mimetic chemicals. The UV dose used was sufficient to saturate the UV excision repair system. Excision repair after combined treatments was estimated by unscheduled DNA synthesis, BrdUrd photolysis, and the loss of sites sensitive to a UV specific endonuclease. Since the repair of damage from UV and its mimetics is coordinately controlled we expected that there would be similar rate-limiting steps in the repair of UV and chemical damage and that after a combined treatment the total amount of repair would be the same as from UV or the chemicals separately. The expectation was not fulfilled. In normal cells repair after a combined treatment was additive whereas in XP cells repair after a combined treatment was usually less than after either agent separately. The chemicals tested were AAAF, DMBA-epoxide, 4NQO, and ICR-170.

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Abbreviations

AAFF:

N-acetoxy-acetylaminoflourene

DMBA:

epoxide – 7, 12 dimethylbenz[a]anthracene 5, 6-oxide

ICR-170:

Acridine mustard

4NQO:

4 nitroquinoline oxide

XP:

Xeroderma pigmentosum

UDS:

Unscheduled DNA synthesis

UV:

Ultraviolet

References

  1. Ahmed, F. E., Gentil, A., Rosenstein, B. S., and Setlow, R. B.: 1980, Biochio, Biophys. Acta., In press.

    Google Scholar 

  2. Ahmed, F. E. and Setlow, R. B.: 1978a, Biophys. J. 24, pp. 665–675.

    Article  CAS  Google Scholar 

  3. Ahmed, F. E. and Setlow, R. B.: 1978b, Biochira. Biophys. Acta 521, pp. 805–817.

    CAS  Google Scholar 

  4. Ahmed, F. E. and Setlow, R. B.: 1979a, Photochem. Photobiol. 29, pp. 983–989.

    Article  CAS  Google Scholar 

  5. Ahmed, F. E. and Setlow, R. B.: 1979b, Cancer Res. 39, pp. 471–479.

    CAS  Google Scholar 

  6. Ahmed, F. E. and Setlow, R. B.: 1980, Chem.-Biol. Interactions, in press.

    Google Scholar 

  7. Arlett, C. F. and Lehnann, A. R.: 1978, Ann. Rev. Genet. 12, pp. 95–111.

    Article  CAS  Google Scholar 

  8. Brown, A. J., Fickel, T. H., Cleaver, J. E., Lohraan, P. H. M., Wade, M. H., and Waters, R.: 1979, Cancer Res. 39, pp. 2522–2527.

    CAS  Google Scholar 

  9. Cerutti, P. A. and Xetrawali, M.: 1979, Radiation Research (Proc. 6th Int. Cong. Radiat. Res., Tokyo ), pp. 423–432.

    Google Scholar 

  10. Cleaver, J. E. and Bootsma, D.: 1975, Ann. Rev. Genet. 9, pp. 19–38.

    Article  CAS  Google Scholar 

  11. Fornace, A. J. and Little, J. B.: 1979, Cancer Res. 39, pp. 704–710.

    CAS  Google Scholar 

  12. Friedberg, E. C., Ehmann, U. K., and Williams, J. I.: 1979, Adv. Radiat. Res. 8, pp. 85–174.

    CAS  Google Scholar 

  13. Grossman, L., Riazuddin, S., Haseltine, W. A., and Lindan, C.: 1978, Cold Spr. Harb. Symp. Quant. Biol. 43, pp. 947–955.

    Google Scholar 

  14. Hanawalt, P. C., Cooper, P. R., Ganesan, A. K., and Smith, C. A.,: 1979, Ann. Rev. Biochem. 48, pp. 783–836.

    Article  CAS  Google Scholar 

  15. Jahn, C. L. and Litman, G. W.: 1979, Biochemistry 18, pp. 1442–1449.

    Article  CAS  Google Scholar 

  16. Kantor, G. J. and Hull, D. R.: 1979, Biophys. J. 27, pp. 359–370.

    Article  CAS  Google Scholar 

  17. Kantor, G. J., Sutherland, J. C., and Setlow, R. B: 1980, Photochem.

    Google Scholar 

  18. Photobiol., in press. Maher, V. M., Dorney, D. J., Mendrala, A. L., Konze-Thomas, B., and McCoraick, J. J.: 1979, Mutat. Res. 62, pp. 311–323.

    Article  CAS  Google Scholar 

  19. Paterson, M. C.: 1978, Adv. Radiat. Biol. 7, pp. 1–53.

    Google Scholar 

  20. Regan, J. D. and Setlow, R. B.: 1974, Cancer Res. 34, pp. 3318–3325.

    CAS  Google Scholar 

  21. Reynolds, R. J., Natarajan, A. T., and Lohman, P. H. M: 1979, Mutat. Res. 64, pp. 353–356.

    CAS  Google Scholar 

  22. Rosenstein, B. S. and Setlow, R. B.: 1980, Photochem. Photobiol., in press.

    Google Scholar 

  23. Rothman, R. H., and Setlow, R. B.: 1979, Photochem. Photobiol. 29, pp. 57–61.

    Article  CAS  Google Scholar 

  24. Scott, E. L. and Straf, M. L.: 1977, Origins of Human Cancer, Cold Spring Harbor Lab., pp. 529–546.

    Google Scholar 

  25. Seeberg, E.: 1978, Proc. Nat. Acad. Sc. USA 75, pp. 2569–2573.

    Article  CAS  Google Scholar 

  26. Setlow, R. B.: 1974, Proc. Nat. Acad. Sci. USA 71, pp. 3363–3366.

    Article  CAS  Google Scholar 

  27. Setlow, R. B.: 1978, Nature 271, pp. 713–717.

    Article  CAS  Google Scholar 

  28. Setlow, R. B.: 1980, Arch. Toxicol.: in press.

    Google Scholar 

  29. Setlow, R. B. and Grist, E.: 1980, 8th Int. Photobiol. Cong., in press.

    Google Scholar 

  30. Setlow, R. B. and Setlow, J. K.: 1972, Ann. Rev. Biophys. Bioengineer. 1, pp. 293–346.

    Article  Google Scholar 

  31. Smerdon, M. J., Kastan, M. B., and Lieberman, M. W.: 1979, Biochemistry 18, pp. 3732–3739.

    Article  CAS  Google Scholar 

  32. Waldstein, E. A., Peller, S., and Setlow, R. B.: 1979, Proc. Nac. Acad. Sci. USA 76, pp. 3746–3750.

    Article  CAS  Google Scholar 

  33. Yarosh, D.: 1979, Amer. Soc. Photobiol. Abstracts 7, p. 157.

    Google Scholar 

  34. Zelle, B. and Bootsma, D.: 1980, Mutat. Res., in press.

    Google Scholar 

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Author information

Author notes

  1. Farid E. Ahmed

    Present address: Pharmacopathics Research Laboratories, Inc., 9705 North Washington Blvd., Laurel, MD, USA, 20810

Authors and Affiliations

  1. Biology Department, Brookhaven National Laboratory, Upton, New York, 11973, USA

    R. B. Setlow & Farid E. Ahmed

Authors
  1. R. B. Setlow
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  2. Farid E. Ahmed
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Editor information

Editors and Affiliations

  1. Institut de Biologie Physico-Chimique (Fondation Edmond de Rothschild), Université Pierre et Marie Curie (Paris VI), Paris, France

    Bernard Pullman

  2. The Johns Hopkins University, Baltimore, Maryland, USA

    Paul O. P. Ts’o

  3. National Cancer Institute, Bethesda, Maryland, USA

    Harry Gelboin

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© 1980 D. Reidel Publishing Company

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Setlow, R.B., Ahmed, F.E. (1980). DNA Repair in Human Cells Exposed to Combinations of Carcinogenic Agents. In: Pullman, B., Ts’o, P.O.P., Gelboin, H. (eds) Carcinogenesis: Fundamental Mechanisms and Environmental Effects. The Jerusalem Symposia on Quantum Chemistry and Biochemistry, vol 13. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-9104-0_37

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  • DOI: https://doi.org/10.1007/978-94-009-9104-0_37

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-009-9106-4

  • Online ISBN: 978-94-009-9104-0

  • eBook Packages: Springer Book Archive

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