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Structural Modifications and Specific Recognition by Antibodies of Carcinogenic Aromatic Amines Bound to DNA

  • Conference paper
Carcinogenesis: Fundamental Mechanisms and Environmental Effects

Part of the book series: The Jerusalem Symposia on Quantum Chemistry and Biochemistry ((JSQC,volume 13))

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Abstract

Local structural modification of the native DNA molecule, induced by the covalent binding to the C-8 of guanine residue of acetylamino- fluorene (nDNA-AAF), was studied previously by means of different physical and biochemical techniques. We present here the results obtained by using two other methods:

  1. 1)

    The local unwinding can be determined from the number of bound carcinogens necessary to transform the supercoiled circular form of DNA to the relaxed one. In the case of nDNA-AAF it amounts to about -20 degrees.

  2. 2)

    Detection and mapping of bound carcinogens can be realized by means of antibodies able to recognize specifically the structural features of the modified regions. With chromatin subunits (core particle and trinucleosomes) the mapping reveals the peculiar accessibility of the linker DNA.

This latter method appears to be applicable to other types of carcinogens and able, after some improvements, to solve several problems related to the initiation step of carcinogenesis.

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Author information

Authors and Affiliations

  1. Department of Biophysics, Institut de Biologie Moléculaire et Cellulaire, 15 rue Descartes, 67084, Strasbourg cédex, France

    M. P. Daune, G. de Murcia, A. M. Freund, R. P. P. Fuchs & M. C. Lang

  2. Centre de Biophysique Moléculaire, 45045, Orléans cédex, France

    M. Leng & E. Sage

Authors
  1. M. P. Daune
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  2. G. de Murcia
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  3. A. M. Freund
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  4. R. P. P. Fuchs
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  5. M. C. Lang
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  6. M. Leng
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  7. E. Sage
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Editor information

Editors and Affiliations

  1. Institut de Biologie Physico-Chimique (Fondation Edmond de Rothschild), Université Pierre et Marie Curie (Paris VI), Paris, France

    Bernard Pullman

  2. The Johns Hopkins University, Baltimore, Maryland, USA

    Paul O. P. Ts’o

  3. National Cancer Institute, Bethesda, Maryland, USA

    Harry Gelboin

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© 1980 D. Reidel Publishing Company

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Daune, M.P. et al. (1980). Structural Modifications and Specific Recognition by Antibodies of Carcinogenic Aromatic Amines Bound to DNA. In: Pullman, B., Ts’o, P.O.P., Gelboin, H. (eds) Carcinogenesis: Fundamental Mechanisms and Environmental Effects. The Jerusalem Symposia on Quantum Chemistry and Biochemistry, vol 13. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-9104-0_16

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  • DOI: https://doi.org/10.1007/978-94-009-9104-0_16

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-009-9106-4

  • Online ISBN: 978-94-009-9104-0

  • eBook Packages: Springer Book Archive

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