Minimal Residual Disease May be Treated by Chessboard Vaccination with Vibrio Cholerae Neuraminidase (VCN) and Tumor Cellss
Vibrio cholerae neuraminidase (VCN) has been shown to have an adjuvant effect predominantly for the cellular immune response when admixed with a variety of antigens. On the basis of these findings, the therapeutic effect of specific immunotherapy with the use of VCN was evaluated on the growth of metastases of Lewis lung adenocarcinoma in C57B1/6 mice. Tumor immunotherapy was performed after complete excision of the primary tumor graft and after an adapted chemotherapy (cyclophosphamide) by intradermal injection of increasing numbers of tumor cells (105 – 107) mixed with increasing amounts of VCN (0–65 mU). This procedure has already been described as “chessboard vaccination”.
Such treatment reduced the number of animals that died due to metastasis. In contrast, subcutaneous injection of VCN-treated but subsequently washed tumor cells had no effect. In therapeutic studies in dogs suffering from spontaneous breast tumors, the s.c. injection of VCN-treated cells had, depending on the cell dose, either a therapeutical effect or no effect at all (low dose), or even an enhancing effect on tumor growth (high dose). With the chessboard vaccination procedure, how ever, a therapeutic effect could be seen which was not cell dose dependent. Thus, chessboard vaccination, which has less risk of inducing tumor enhancement, may be recommended for tumor immunotherapy.
KeywordsToxicity Leukemia Adenocarcinoma CaCl Trypsin
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