Advertisement

Metastasis pp 163-168 | Cite as

In Vivo and in Vitro Selection of Experimental Metastatic Variants of Rodent Melanoma, Mammary Carcinoma and Lymphosarcoma

  • G. L. Nicolson
  • A. Neri
  • C. L. Reading
  • K. M. Miner
Part of the Developments in Oncology book series (DION, volume 4)

Abstract

Metastasis via lymphatics and blood does not always result in tumor colonization of tissues and organs based strictly on anatomical considerations (Salsbury, 1975; Sugarbaker, 1952). Many blood-borne experimental metastatic tumor systems show nonrandom colonization patterns that do not correlate with the initial capillary beds encountered (Dunn et al., 1954; Parks, 1974; Fidler and Nicolson, 1976, 1977; Brunson and Nicol son, 1978, 1979; Conley, 1979). After their initial arrest, malignant cells may die, invade and grow or detach and recirculate to other sites (Zeidman, 1961; Fisher and Fisher, 1967; Fidler and Nicolson, 1976, 1977; Brunson and Nicolson, 1978, 1979) suggesting that factors other than non-specific trapping in the microcirculation determine secondary site location (Nicol son, 1978). In order to determine the host and tumor cell properties important in metastatic tumor spread and survival at distant sites, we have developed animal tumor models in syngeneic hosts that show distinct metastatic behaviors. Several of these models have been based on sequential selection of murine B16 melanoma lines for enhanced blood-borne implantation, survival and growth to obtain sublines capable of preferentially colonizing lungs (Fidler, 1973), brain (Brunson et al., 1978) or ovary (Brunson and Nicol son, 1979). Similarly we have selected in vivo murine RAW117 lymphosarcoma sublines that show enhanced liver colonization after intravenous (i.v.) administration (Brunson and Nicolson, 1978) and rat 13762 mammary adenocarcinoma sublines that show colonization of regional lymph nodes and/or lungs after implantation subcutaneously (s.c.) (Neri et al., 1979). In addition, it has been possible to select tumor cell variant sublines in vitro for loss of sensitivity to cell-mediated toxicity (Fidler et al., 1976), decreased binding to immobilized-lectins (Reading et al., 1980) and increased invasion of tissues (Hart, 1980) or veins (Poste et al., 1980).

Keywords

Wheat Germ Agglutinin Variant Line Experimental Metastasis Sequential Selection Peanut Agglutinin 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Brunson, K.W. and Nicolson, G.L., 1978. Selection and biologic properties of malig- nant variants of a murine lymphosarcoma. J. Natl. Cancer Inst., 61: 1499–1503.PubMedGoogle Scholar
  2. Brunson, K.W. and Nicol son, G.L., 1979. Selection of malignant melanoma variant cell lines for ovary colonization. J. Supramol. Struct., 11: 517–528.PubMedCrossRefGoogle Scholar
  3. Brunson, K.W. and Nicolson, G.L., 1980. Experimental brain metastasis. In: L. Weiss, H. Gilbert and J.B. Posner (Editors), Brain Metastasis, G.K. Hall and Co., Boston, pp. 50–65.CrossRefGoogle Scholar
  4. Brunson, K.W., Beattie, G. and Nicolson, G.L., 1978. Selection and altered tumour cell properties of brain-colonising metastatic melanoma. Nature, 272: 543–545.PubMedCrossRefGoogle Scholar
  5. Conley, F.K., 1979. Development of a metastatic brain tumor model in mice. Cancer Res., 39: 1001–1007.PubMedGoogle Scholar
  6. Dunn, T.B., 1954. Normal and pathologic anatomy of the reticular tissue in laboratory mice, with a classification and discussion of neoplasms. J. Natl. Cancer Inst., 14: 1281–1433.PubMedGoogle Scholar
  7. Fidler, I.J., 1973. Selection of successive tumor lines for metastasis. Nature New Biol., 242:. 148–149.Google Scholar
  8. Fidler, I.J. and Kripke, M.L., 1977. Metastasis results from pre-existing variant cells within a malignant tumor. Science, 197: 893–895.PubMedCrossRefGoogle Scholar
  9. Fidler, I.J. and Nicolson, G.L., 1976. Organ selectivity for implantation, survival and growth of B16 melanoma variant tumor lines. J. Natl. Cancer Inst., 57: 1199–1202.PubMedGoogle Scholar
  10. Fidler, I.J. and Nicolson, G.L., 1977. Fate of recirculating Bl6 melanoma metastatic variant cells in parabiotic syngeneic recipients. J. Natl. Cancer Inst., 58: 1867–1872.Google Scholar
  11. Fidler, I.J., Gersten, D.M. and Budmen, M.B., 1976. Characterization in vivo and in vitro of tumor cells selected for resistance to syngeneic lymphocyte-mediated cytotoxicity. Cancer Res., 36: 3160–3165.PubMedGoogle Scholar
  12. Fisher, B. and Fisher, E.R., 1967. The organ distribution of disseminated 51Crlabeled tumor cells. Cancer Res., 27: 412–420.PubMedGoogle Scholar
  13. Hart, I.R., 1979. The selection and characterization of an invasive variant of B16 melanoma. Am. J. Pathol., 97: 587–600.PubMedGoogle Scholar
  14. Kripke, M.L., Gruys, E. and Fidler, I.J., 1978. Metastatic heterogeneity of cells from an ultraviolet light-induced murine fibrosarcoma of recent origin. Cancer Res., 38: 2962–2967.PubMedGoogle Scholar
  15. Neri, A., Ruoslahti, E. and Nicolson, G.L., 1979. Relationship of fibronectin to th metastatic behavior of rat mammary adenocarcinoma cell lines and clones. J. Supr mol. Struct (suppl. 3 ), p. 181.Google Scholar
  16. Nicolson, G.L., 1978. Experimental tumor metastasis: Characteristics and organ specificity. BioScience, 28: 441–447.Google Scholar
  17. Novogrodsky, A., Lotan, R., Ravid, A. and Sharon, N., 1975. Peanut agglutinin, a ne mitogen that binds to galactosyl sites exposed after neuraminidase treatment. J. Immunol., 115: 1243–1248.PubMedGoogle Scholar
  18. Parks, R.C., 1974. Organ-specific metastasis of a transplantable reticulum cell sarcoma. J. Nati. Cancer Inst., 52: 971–973.Google Scholar
  19. Poste, G., Doll, J., Hart, I.R. and Fidler, I.J., 1980. In vitro selection of murin B16 melanoma variants with enhanced tissue invasive properties. Cancer Res., in press, May, 1980 issue.Google Scholar
  20. Reading, C.L., Brunson, K.W., Torrianni, M. and Nicolson, G.L., 1980. Malignancies of murine lymphosarcoma correlate with decreased cell surface display of RNA-tumor virus envelope glycoprotein gp70. Proc. Natl. Acad. Sci. U.S.A., in press.Google Scholar
  21. Salsbury, A.J., 1975. The significance of the circulating cancer cell. Cancer Treatment Rev., 2: 55–72.CrossRefGoogle Scholar
  22. Sugarbaker, E.V., 1952. The organ selectivity of experimentally induced metastasis in rats. Cancer, 5: 606–612.PubMedCrossRefGoogle Scholar
  23. Zeidman, I., 1961. The fate of circulating tumor cells. I. Passage of cells through capillaries. Cancer Res., 21: 38–39.PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 1980

Authors and Affiliations

  • G. L. Nicolson
  • A. Neri
  • C. L. Reading
  • K. M. Miner

There are no affiliations available

Personalised recommendations