Skip to main content

Addition of Pharmacological Agents to the Ejaculate

  • Chapter
Homologous Artificial Insemination (AIH)

Part of the book series: Clinics in Andrology ((CLAN,volume 1))

  • 94 Accesses

Abstract

Knowledge of the anatomical mechanisms of sperm motility has come a long way from the homunculus theory suggested by Hartsoecker in 1698. Electron microscopic studies have shown that the motility of human spermatozoa is provided in the axial spiral complex of the tail (Pedersen 1970) by a sliding mechanism of the axonemal microtubules with respect to one another under the gripping action of the outer and inner dynein arms (Huxley 1969). This produces contractions and relaxations in the tail, propelling the spermatozoon in a quasipurposeful manner, a self-propulsion that must suffice if it is to achieve contact with the released ovum after the initial extra impetus given by the neuromuscular movements of the male reproductive tract and the possible copulatory motor activity of the female genital tract have finished.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 39.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 54.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Atkinson EE, Walton GE: Adenosine triphosphate concentration in metabolic regulation. J Biol Chem 242: 3239, 1967.

    PubMed  CAS  Google Scholar 

  • Barkay J, Zuckerman H: Paper presented at the first international symposium on AIH and male subfertility. Bordeaux, 1978.

    Google Scholar 

  • Barkay J. Zuckerman H: Paper presented at the first international symposium on AIH and male subfertility. Bordeaux, 1979.

    Google Scholar 

  • Bartak V: Sperm velocity test in clinical practice. Jut J Fertil 16: 107, 1971.

    CAS  Google Scholar 

  • Behrman SJ: Preservation of human sperm by liquid nitrogen vapor freezing. In: Current problems in fertility, Ingelman-Sundberg A, Lunell NO (eds), New York, Plenum, 1971.

    Google Scholar 

  • Behrman SJ, Ackerman DR:Freeze preservation of human spermatozoa. Am J Obstet Gynecol 103: 654, 1969.

    PubMed  CAS  Google Scholar 

  • Bunge RG: Caffeine stimulation of ejaculated human spermatozoa. Urology 1: 371, 1973.

    Article  PubMed  CAS  Google Scholar 

  • Bygdeman M: Prostaglandins in human seminal fluid and their correlation to fertility. Int J Fertil 14: 228, 1969.

    PubMed  CAS  Google Scholar 

  • Bygdeman M, Fredricsson B, Svanborg K, Samuelsson B: The relation between fertility and prostaglandin content of seminal fluid in man. Fertil Steril 21: 622. 1970.

    PubMed  CAS  Google Scholar 

  • Cohen MS, Colin MJ, Golimbu M, Hotchkiss RS: The effects of prostaglandins on sperm motility. Fertil Steril 28: 78, 1977.

    PubMed  CAS  Google Scholar 

  • Collier JG, Flower RJ, Stanton SL: Seminal prostaglandins in infertile men. Fertil Steril 26: 868, 1975.

    PubMed  CAS  Google Scholar 

  • Cooper TG, Orgebin-Crist MD: The effect of epididiymal and testicular fluid on the fertilizing capacity of testicular and epididymal spermatozoa. Andrologia 7: 85, 1975.

    Article  PubMed  CAS  Google Scholar 

  • Damas J, Deby C: Libération de prostaglandines par la bradykinine chez le rat. C R Soc Biol 168: 375, 1974.

    CAS  Google Scholar 

  • Dougherty KA, Cockett A, Urry R: Caffeine, Theophylline, and human sperm motility. Fertil Steril 27: 541, 1976.

    PubMed  CAS  Google Scholar 

  • Drevius LO: The sperm rise test. J Reprod Fert 24: 427, 1971.

    Article  CAS  Google Scholar 

  • Duijn C van, Lierop JH van: Effect of temperature on photosensitivity of spermatozoa. Nature 201: 5055, 1966.

    Google Scholar 

  • Eliasson R, Lindholmer C: Distribution and properties of spermatozoa in different fractions of split ejaculates. Fertil Steril 23: 252, 1972.

    PubMed  CAS  Google Scholar 

  • Eskin BA, Azarbal S, Sepic R, Slate WG: In vitro responses of the spermatozoa-cervical mucus system treated with prostaglandin-F2a. Obstet Gynecol 41: 436, 1973.

    PubMed  CAS  Google Scholar 

  • Fair W, Clarke R, Wehner N: A correlation of seminal polyamine levels and semen analysis in the human. Fertil Steril 23: 38, 1972.

    PubMed  CAS  Google Scholar 

  • Ferreira SH, Moncada S, Vane JR: Prostaglandins and signs and symptoms of inflammation. In: Prostaglandin synthetase inhibitors, Robinson HJ, Vane JR (eds). New York, Raven, 1974, p 175.

    Google Scholar 

  • Fjallbrant B: Interrelation between high levels of sperm antibodies, reduced penetration of cervical mucus by spermatozoa and sterility in men. Acta Obstet Synecol Scand 47: 102, 1968.

    Article  CAS  Google Scholar 

  • Fjallbrant B, Ackerman DR: Cervical mucus penetration in vitro by fresh and frozen-preserved human semen specimens, J Reprod Fertil 20: 515, 1969.

    Article  PubMed  CAS  Google Scholar 

  • Fritz H, Schiessler H, Schill WB, Tscheache H, Heimburger N, Wallner O: Low molecular weight proteinase (acrosin) inhibitors from human and boar seminal plasma and spermatozoa and human cervical mucus: isolation, properties, and biological aspects. In: Proteases and biological control, Reich E, Rifkin DB, Shaw E (eds), Cold Spring Harbor Laboratory, 1975.

    Google Scholar 

  • Gadden P: Sperm maturation in the male reproductive tract: development of motility. Anat Rec 161: 471, 1968.

    Article  Google Scholar 

  • Garbers DL, First NL, Sullivan JT, Lardy HA: Stimulation and maintenance of ejaculated bovine spermatozoa respiration and motility by caffeine. Biol Reprod 5: 336, 1971.

    PubMed  CAS  Google Scholar 

  • Gryglewski RJ: Structure-activity relationships of some prostaglandin synthetase inhibitors. In: Prostaglandin synthetase inhibitors, Robinson HJ, Vane JR (eds), New York, Raven, 1974, p 33.

    Google Scholar 

  • Haesungcharern A, Chulavatnatol M: Stimulation of human spermatozoal motility by caffeine. Fertil Steril 27: 662, 1973.

    Google Scholar 

  • Hardman JG, Robinson GA, Sutherland EW: Cyclic nucleotides. Annual Review of Physiol 33: 331, 1971.

    Google Scholar 

  • Harrison RF: Insemination of husband’s semen with and without addition of caffeine. Fertil Steril 29: 532, 1978.

    PubMed  CAS  Google Scholar 

  • Hawkins DF: In: Symposium of the Worcester foundation for experimental biology, Ramwell PW, Shaw JE (eds), New York, Interscience, 1967, p. 1.

    Google Scholar 

  • Hicks JJ, Pedron N, Martinez-Manautoi J, Rodado A: Metabolic changes in human spermatozoa related to capacitation. Fertil Steril 23: 172, 1972a.

    PubMed  CAS  Google Scholar 

  • Hicks JJ, Pedron N, Rosado A: Modifications of human spermatozoa glycolysis by cyclic adenosine monophosphate (CAMP), estrogens and follicular fluid. Fertil Steril 23: 886, 1972b.

    PubMed  CAS  Google Scholar 

  • Hoskins DD, Casillas ER: Function of cyclic nucleotides in mammalian spermatozoa. In: Handbook of physiology, volume 5, section 7: Endocrinology of the reproductive system: male, Greepro L, Astwood EB (eds), Washington DC, American Physiological Society, 1975.

    Google Scholar 

  • Huacuja L, Sosa A, Delgrado NM, Rosado A: Kinetic study of the participation of zinc in human spermatozoa metabolism. Life Sci 13: 1383, 1973.

    Article  PubMed  CAS  Google Scholar 

  • Hunt WL, Zaneveld LJD: Prostaglandin effects on sperm penetration of cervical mucus. Abstract 31, international conference of andrology, Detroit, 1975.

    Google Scholar 

  • Huxley HE: Mechanism of muscular contraction. Science 164: 1356, 1969.

    Article  PubMed  CAS  Google Scholar 

  • Jungling ML, Bunge RG: The treatment of spermatogenic arrest with arginine. Fertil Steril 27: 282, 1976.

    PubMed  CAS  Google Scholar 

  • Keller DW, Polakoski KL: L-arginine stimulation of human sperm motility in vitro. Biol Reprod 13: 154, 1975.

    Article  PubMed  CAS  Google Scholar 

  • Krampitz G, Doepfmer, R: Determination of free amino-acids in human ejaculate by ion exchange chromatography. Nature 194: 684, 1962.

    Article  PubMed  CAS  Google Scholar 

  • Kroeks M, Kremer J: The pH in the lower third of the genital tract. In: The uterine cervix in reproduction, Insler V, Bettendorf G, Stuttgart, Georg Thieme, 1977, p 109.

    Google Scholar 

  • Leidl W, Prinzen R, Schill W-B, Fritz H: The effect of kallikrein on motility and metabolism of spermatozoa in vitro. In: Kininogenases, kallikrein: second symposium on physiological properties and pharmacological rationale, Haberland GL, Rohen JW, Schirren C, Huber P (eds), Stuttgart, Schattauer, 1975, p 33.

    Google Scholar 

  • MacLeod J: Semen quality in 1,000 men of known fertility and 800 of infertile marriages. Fertil Steril 2: 115, 1951.

    PubMed  CAS  Google Scholar 

  • MacLeod J, Freund M: Influence of spermatozoa concentration and initial fructose level on fructolysis of human semen. J Appl Physiol 13: 501, 1958.

    PubMed  CAS  Google Scholar 

  • Mann T: Biochemistry of semen and of the male reproductive tract ( 2nd edition ), New York, John Wiley and Sons, 1964.

    Google Scholar 

  • Marrazzi MA. Matschinsky M: Properties of 15-hydroxyprostaglandin dehydrogenase: structural requirements for binding Prostaglandins 1: 373, 1972.

    Article  PubMed  CAS  Google Scholar 

  • Marsh JM, LeMaire WJ: Cyclic AMP accumulation and steroidogenesis in the human corpus luteum: effect of gonadotropins and prostaglandins. J. Clin Endocrinol Metab 39: 99, 1974.

    Article  Google Scholar 

  • Mitchell J, Nelson L, Hafez ESE: Motility of spermatozoa. In: Human semen infertility regulation in men, Hafez ESE (ed), St. Louis, Mosby, 1976.

    Google Scholar 

  • Nevo A: Relations between motility and respiration in human spermatozoa. Rep Fert 16: 351, 1966.

    Google Scholar 

  • Newton AA, Rothschild L: Energy-rich phosphate compounds in bull semen: comparison of their metabolism with anaerobic heat production and impedance change frequency. Proc Royal Soc Lond (Biol) 155: 183, 1961.

    Article  Google Scholar 

  • Norman C, Goldberg E, Porterfield I: The effect of visible radiation on the functional life span of mammalian and avian sperm. Exp Cell Res 28: 69, 1962.

    Article  PubMed  CAS  Google Scholar 

  • Palm S, Fritz H: Kinases in cervical mucus and seminal plasma. In: Kininogenases, Kallekrein, second symposium on physiological properties and pharmacological rationale, Haberland GL, Rohen JW, Schirren C, Huber P (eds), Stuttgart, Schattauer, 1975.

    Google Scholar 

  • Palm S, Schill. W-B, Wallner O, Prinzen R, Fritz H: Occurrence of components of the kallikreinkinin system in human genital tract secretions and their possible function in stimulation of sperm motility and migration. In: Kinins: pharmacodynamics and biological roles, Sicuteri F, Back A, Haberland GL (eds), New York, Plenum, 1976. p. 271.

    Google Scholar 

  • Paulson JD, Parrish RF, Polakowski KL: Comparative synthetic substrate kinetics of porcine acrosin and porcine ß-trypsin. Im J Bio 10: 247. 1979.

    CAS  Google Scholar 

  • Pedersen H: Observations of the axial filament complex of the human spermatozoon. J Ultra-structures 33: 451, 1970.

    Article  CAS  Google Scholar 

  • Petersen RN, Freund M: ATP synthesis and oxidative metabolism in human spermatozoa. Biol Reprod 3: 47, 1970.

    Google Scholar 

  • Petersen RN, Freund M: Effects of H+ and certain membrane-active drugs on glycolysis, motility and ATP Biol Reprod 8: 350, 1973.

    Google Scholar 

  • Polakoski K, Zaneveld L: Biochemical examination of the human ejaculate. In: Techniques of human andrology, Hafez ESE (ed), Amsterdam, North-Holland Biomedical Press, 1977, p 265.

    Google Scholar 

  • Polakoski KL, Zahler WL, Paulson JD: Demonstration of proacrosin and quantitation of acrosin in ejaculated human spermatozoa. Fertil Steril 28: 688, 1977.

    Google Scholar 

  • Poulos A, White I: Phospholipid composition of human spermatozoa and seminal plasma. J Reprod Fertil 35: 265, 1973.

    Article  PubMed  CAS  Google Scholar 

  • Ramwell P, Shaw H: The biological significance of the prostaglandins. Ann N Y Acad Sci 180: 10, 1970.

    Article  Google Scholar 

  • Rosado A, Hicks JJ, Reyes A, Blanco J: Capacitation in vitro of rabbit spermatozoa with cyclic adenosine monophosphate and human follicular fluid. Fertil Steril 25: 82, 1974.

    Google Scholar 

  • Rosado A, Huacuja L, Delgardo N, Pancardo R: Cyclic AMP receptors in the human spermatozoa membrane. Life Sci 17: 1707, 1976.

    Article  Google Scholar 

  • Schachter A, Goldman JA, Zuckerman Z: Treatment of oligospermia with the amino acid arginine J Uro1 110: 311, 1973.

    CAS  Google Scholar 

  • Schill W-B: Humane Spermakonservierung and therapeutische Ausblicke. Hautarzt 23: 525, 1972.

    PubMed  CAS  Google Scholar 

  • Schill W-B: Increased fructolysis of kallikrein-stimulated human spermatozoa. Andrologia 7: 105, 1975a.

    Article  PubMed  CAS  Google Scholar 

  • Schill W-B: Stimulation of sperm motility by kinins in fresh and 24 hours aged human ejaculates. Andrologia 7: 135, 1975b.

    Article  PubMed  CAS  Google Scholar 

  • Schill W-B: Caffeine and kallikrein-induced stimulation of human sperm motility: a comparative study. Andrologia 7: 229, 1975e.

    Article  PubMed  CAS  Google Scholar 

  • Schill W-B: Influence of the kallikrein-kinin system on human sperm motility in vitro. In: Kininogenase, second symposium on physiological properties and pharmacological rationale, kallikrein Haberland GL, Rohen JW, Schirren C, Huber P (eds), Stuttgart. Schattauer, 1975d.

    Google Scholar 

  • Schill W-B: Kallikrein as a therapeutical means in the treatment of male infertility. In: Kininogenases, fourth sympasiwn on physiological properties and pharmacological rationale, kallikrein: Haberland GL, Rohen JW, Suzuki T (eds), Stuttgart, Schattauer, 1977, p 251.

    Google Scholar 

  • Schill W-B: Effect of kallikrein on the motility of fresh and frozen human semen. Presented to the first international symposium on AIH and male subfertility, Bordeaux, 1978.

    Google Scholar 

  • Schill W-B, Haber land GL: Kinin-induced enhancement of sperm motility. Hoppe-Seyler’s Z Physiol Chem 355: 299, 1974a.

    Google Scholar 

  • Schill W-B, Haberland GL: In vitro stimulation of human sperm motility by kallikrein. World Cong Fertil Steril8:153, 1974b.

    Google Scholar 

  • Schill W-B, Preissler G: Improvement of cervical mucus spermatozoal penetration by kinins: a possible therapeutical approach in the treatment of male subfertility. In: The uterine cervix in reproduction, Insler V, Bettendorf G (eds), Stuttgart. Georg Thieme, 1977. p 134.

    Google Scholar 

  • Schill W-B, Pritsch W: Kinin-induced enhancement of sperm motility in cryo-preserved human spermatozoa. Proc Int Congress Animal Reprod Artif insemination 8: 1071, 1976.

    Google Scholar 

  • Schill W-B, Pritsch W, Preissler G: Effect of caffeine and kallikrein on cryopreserved human spermatozoa. Int J Fertil 24: 27, 1979.

    PubMed  CAS  Google Scholar 

  • Schill W-B, Braun-Falco O, Haberland GL: The possible role of kinins in sperm motilities. Int J Fetid 19: 163, 1974.

    CAS  Google Scholar 

  • Schill W-B, Leidl W, Prinzen R, Wallner O: Stimulation of sperm motility and sperm metabolism by kallikrein, a kinin-liberating proteinase. Proc European Sterility Congres 4: 375, 1975.

    Google Scholar 

  • Schill W-B, Wallner O, Palm S, Fritz H: Kinin stimulation of spermatozoa motility and migration in cervical mucus. In: Human semen and fertility regulation in men, Hafez ESE (ed), St. Louis Mosby, 1976, p 442.

    Google Scholar 

  • Schirren C: Experimental studies on the influence of kallikrein on human sperm motility in vitro. In:Kininoginases, kallikrein: second symposium on physiological properties and pharmacological rationale, Haberland GL, Rohen JW, Schirren C, Huber P (eds), Stuttgart, Schattauer 1975, p 59.

    Google Scholar 

  • Schoenfeld C, Amelar RD, Dubin L: Stimulation of ejaculated human spermatozoa by caffeine: a preliminary report. Fertil Steril 24: 772, 1973.

    PubMed  CAS  Google Scholar 

  • Schoenfeld C, Amelar RD, Dubin L: Stimulation of ejaculated human spermatozoa by caffeine. Fertil Steril 26: 158, 1975.

    PubMed  CAS  Google Scholar 

  • Sherman JK: Synopsis of the use of frozen human semen since 1964: state of the art of human semen banking. Fertil Steril 24: 397, 1973.

    PubMed  CAS  Google Scholar 

  • Steiner R, Hofmann N, Hartmann R: The influence of kallikrein on the velocity of human spermatozoa measured by Laser-Doppler spectroscopy. In: Kininogenases, kallikrein: fourth symposium on physiological properties and pharmacological rationale, Haberland GL, Rohen JW, Suzuki T (eds), Stuttgart, Schattauer, 1977, p 229.

    Google Scholar 

  • Tash JA, Mann FRC: Adenosine 3’ 5’ cyclic monophosphate in relation to motility and senescence of spermatozoa. Proc Royal Soc Lond (Biol) 184: 109, 1973.

    Article  CAS  Google Scholar 

  • Ulstein M: Sperm penetration of cervical mucus as a criterion of male fertility. Acta Obstet Gynecol Scand 51: 335, 1972.

    Article  PubMed  CAS  Google Scholar 

  • Ulstein M: Fertility of donors at heterologous insemination. Acta Obstet Gynecol Scand 52: 97, 1973.

    Article  PubMed  CAS  Google Scholar 

  • Wallner O, Schill W-B, Brosser A, Fritz H: Participation of the kallikrein-kinin system in sperm penetration through cervical mucus: in vitro studies. In: Kininogenases, kallikrein: second symposium on physiological properties and pharmacological rationale, Haberland GL. Rohen JW, Schirren C, Huber P (eds), Stuttgart, Schattauer, 1975, p 63.

    Google Scholar 

  • Wolf P, Blasco L, Khan MA, Litt M: Human Cervical mucus II: changes in viscoelasticity during the ovulatory menstrual cycle. Fertil Steril 28: 47, 1977.

    PubMed  CAS  Google Scholar 

  • Zaneveld L, Polakoski K: Collection and the physical examination of the ejaculate. In: Techniques in human andrology, Hafez ESE (ed), Amsterdam, North-Holland Biomedical Press, 1977, p 159.

    Google Scholar 

Download references

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 1980 Martinus Nijhoff Publishers bv, The Hague

About this chapter

Cite this chapter

Paulson, J.D., Harrison, R., Schill, W.B., Barkay, J. (1980). Addition of Pharmacological Agents to the Ejaculate. In: Emperaire, J.C., Audebert, A., Hafez, E.S.E. (eds) Homologous Artificial Insemination (AIH). Clinics in Andrology, vol 1. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-8817-0_7

Download citation

  • DOI: https://doi.org/10.1007/978-94-009-8817-0_7

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-009-8819-4

  • Online ISBN: 978-94-009-8817-0

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics