The Locomotor Behaviour of Human Blood Monocytes in Chemotactic and Chemokinetic Environments and the Role of Substratum in Monocyte Locomotion

  • P. C. Wilkinson
  • R. B. Allan
  • A. Blussé van Oud Alblas


Mononuclear phagocytes from a variety of sources, for example, blood monocytes, macrophages from peritoneal exudates or from the unstimulated peritoneal cavity, and alveolar macrophages, all show locomotor reactions to chemoattractants. In this paper we deal chiefly with blood monocytes. Although the role of macrophage chemotaxis in the evolution of inflammatory lesions is uncertain, it does at least seem probable that the monocytes which leave the blood are in the first instance directed into such lesions by chemotactic stimuli.


Mononuclear Phagocyte Chemotactic Factor Positive Gradient Monocyte Chemotaxis Filter Assay 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Allan, R.B. & P.C. Wilkinson (1978) A visual analysis of chemotactic and chemokinetic locomotion of human neutrophil leucocytes. Experimental Cell Research 111, 191.PubMedCrossRefGoogle Scholar
  2. Bumol, T.F. & S.D. Douglas (1977) Human monocyte cytoplasmic spreading in vitro: Early kinetics and scanning electron microscopy. Cellular Immunology 34, 70.PubMedCrossRefGoogle Scholar
  3. Carrel, A. & A.H. Ebeling (1926) The fundamental properties of the fibro-blast and the macrophage. II. The macrophage. Journal of Experimental Medicine 44, 285.PubMedCrossRefGoogle Scholar
  4. Carter, S.B. (1965) Principles of cell movement: the direction of cell movement and cancer invasion. Nature 208, 1183.PubMedCrossRefGoogle Scholar
  5. Carter, S.B. (1967) Haptotaxis and the mechanism of cell motility. Nature 213, 256.PubMedCrossRefGoogle Scholar
  6. Dierich, M.P., Wilhelmi, D. & G. Till (1977) Essential role of surface bound chemoattractant in leukocyte migration. Nature 270, 351.PubMedCrossRefGoogle Scholar
  7. Dixon, H.M. & M. McCutcheon (1935) Absence of chemotropism in lymphocytes. Archives of Pathology 19, 679.Google Scholar
  8. Fraenkel, G.S. & D.L. Gunn (1961) The orientation of animals; Kineses, taxes and compass reactions. Dover, New York.Google Scholar
  9. Gail, M.H. & C.W. Boone (1970) The locomotion of mouse fibroblasts in tissue culture. Biophysical Journal 10, 980.PubMedCrossRefGoogle Scholar
  10. Griffin, F.M., Griffin, J.A., Leider, J.E. & S.C. Silverstein (1975) Studies on the mechanism of phagocytosis. I. Requirements for circumferential attachment of particle-bound ligands to specific receptors on the macrophage plasma membrane. Journal of Experimental Medicine 142, 1263.PubMedCrossRefGoogle Scholar
  11. Griffin, F.M., Griffin, J.A., & S.C. Silverstein (1976) Studies on the mechanism of phagocytosis. II. The interaction of macrophages with anti-immunoglobulin IgG-coated bone-marrow-derived lymphocytes. Journal of Experimental Medicine 144, 788.PubMedCrossRefGoogle Scholar
  12. Harris, H. (1953) Chemotaxis of monocytes. British Journal of Experimental Pathology 34, 276.PubMedGoogle Scholar
  13. Henney, C.S. & K. Ishizaka (1968) Antigenic determinants specific for aggregated yG-globulin. Journal of Immunology 100, 718.Google Scholar
  14. Keller, H.U., Wilkinson, P.C., Abercrombie, M., Becker, E.L., Hirsch, J.G., Miller, M.E., Ramsey, W.S. & S.H. Zigmond (1977a) A proposal for the definition of terms related to locomotion of leucocytes and other cells. Clinical and Experimental Immunology 27, 377.PubMedGoogle Scholar
  15. Keller, H.U., Wissler, J.H., Hess, M.W. & H. Cottier (1977b) Relation between stimulus intensity and neutrophil chemotactic response. Experientia 33, 534.PubMedCrossRefGoogle Scholar
  16. Keller, H.U., Wissler, J.H., Hess, M.W. & H. Cottier (1978) Distinct chemo-kinetic and chemotactic responses in neutrophil granulocytes. European Journal of Immunology 8, 1.PubMedCrossRefGoogle Scholar
  17. Lewis, W.H. (1934) On the locomotion of the polymorphonuclear neutrophils of the rat in autoplasma cultures. Bulletin of the Johns Hopkins Hospital 55, 273.Google Scholar
  18. Lewis, W.H. (1939) The role of a superficial plasmagel layer in changes of form, locomotion and division of cells in tissue cultures. Archiv für Experimentelle Zellforschung 23, 1.Google Scholar
  19. McCutcheon, M. (1946) Chemotaxis in leukocytes. Physiological Reviews 26, 319.PubMedGoogle Scholar
  20. Nossal, R. & S.H. Zigmond (1976) Chemotropism indices for polymorphonuclear leukocytes Journal 16, 1171.Google Scholar
  21. Ramsey, W.S. (1972) Locomotion of human polymorphonuclear leucocytes. Experimental Cell Research 72, 489.PubMedCrossRefGoogle Scholar
  22. Robineaux, R. (1964) Movements of cells involved in inflammation and immunity. In: R.D. Allen & N. Kamiya (eds) Primitive Motile Systems in Cell Biology, p. 351. Academic Press, New York.CrossRefGoogle Scholar
  23. Rothert, W. (1901) Beobachtungen und Betrachtungen über taktische Reiz-erscheinüngen. Flora 88, 371.Google Scholar
  24. Rydgren, L., Norberg, B., Svensson, B. & G. Simmingskoeld (1978) The phagocytosis associated chemotaxis of human mononuclear leucocytes from peripheral blood is not antitubulin-sensitive. Scandinavian Journal of Immunology 7, 251.PubMedCrossRefGoogle Scholar
  25. Senda, N., Tamura, H., Shibata, N., Yoshitake, J., Kondo, K. & K. Tanaka (1975) The mechanism of the movement of leucocytes. Experimental Cell Research 91, 393.PubMedCrossRefGoogle Scholar
  26. Wilkinson, P.C. (1973) Recognition of protein structure in leukocyte chemotaxis. Nature 244, 512.PubMedCrossRefGoogle Scholar
  27. Wilkinson, P.C. (1976) A requirement for albumin as carrier for low molecular weight leukocyte chemotactic factors. Experimental Cell Research 103, 415.PubMedCrossRefGoogle Scholar
  28. Wilkinson, P.C. & R.B. Allan (1978a) Assay systems for measuring leukocyte locomotion: an overview. In: J.I. Gallin & P.G. Quie (eds) Leukocyte chemotaxis, p. 1–24. Raven Press, New York.Google Scholar
  29. Wilkinson, P.C. & R.B. Allan (1978b) Chemotaxis of neutrophil leukocytes towards substratum-bound protein attractants. Experimental Cell Research, 117, 403.PubMedCrossRefGoogle Scholar
  30. Wilkinson, P.C. & I.C. McKay (1974) Recognition in leucocyte chemotaxis. Studies with structurally modified proteins. Antibiotics and chemotherapy 19, 421.PubMedGoogle Scholar
  31. Wilkinson, P.C., Parrott, D.M.V., Russell, R.J. & F. Sless (1977) Antigen-induced locomotor responses in lymphocytes. Journal of Experimental Medicine 145, 1158.PubMedCrossRefGoogle Scholar
  32. Zigmond, S.H. (1977) Ability of polymorphonuclear leukocytes to orient in gradients of chemotactic factors. Journal of Cell Biology 75, 606.PubMedCrossRefGoogle Scholar
  33. Zigmond, S.H. (1978) Chemotaxis by polymorphonuclear leukocytes. Journal of Cell Biology 77, 269.PubMedCrossRefGoogle Scholar
  34. Zigmond, S.H. & J.G. Hirsch (1973) Leukocyte locomotion and chemotaxis. New methods for evaluation and demonstration of cell-derived chemotactic factor. Journal of Experimental Medicine 137, 387.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 1980

Authors and Affiliations

  • P. C. Wilkinson
  • R. B. Allan
  • A. Blussé van Oud Alblas

There are no affiliations available

Personalised recommendations