SOP/DTX/150 Assay of DNA Repair Using [3H]-Deoxyguanosine

  • I. P. Sword
  • Ritchie Thomson

Abstract

This assay is complementary to that described in SOP/DTX/149. Many chemicals preferentially damage purine residues within DNA particularly guanine residues. The repair of such damage can involve the replacement of only a few nucleotides at the site of the lesion. In many cases therefore, such repair may not involve the turnover of thymidine residues and hence will not be detected by the technique discussed in SOP/DTX/149. Thus, an assay using [3H]-deoxyguanosine is used when results by the first assay are negative or give doubtful positive responses.

Keywords

Toxicity Phenol EDTA Trypsin Paraffin 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Copyright information

© Inveresk Research International Limited 1980

Authors and Affiliations

  • I. P. Sword
    • 1
  • Ritchie Thomson
    • 1
    • 2
  1. 1.Inveresk Research International LimitedEdinburghScotland
  2. 2.Sterling MediterraneanAttikiGreece

Personalised recommendations