Abstract
The role of mucus in the immune expulsion of Nippostrongylus brasiliensis from the small intestine of the rat was examined. A glycoprotein isolated from intestinal secretions by Sepharose 4B chromatography was characterised by incorporation of (35S) - Na2SO4 and D-(1-14C)glucosamine, immure-diffusion, polyacrylamide gel electrophoresis, and by its immure-histochemical localisation in goblet cells. These results suggested that the glycoprotein was very similar to a soluble rat intestinal mucin characterised by Forstner et al., 1973. The rate of incorporation of D-(1-14C) glucosamine into acid- precipitable material in intestinal secretions was significantly increased in rats harbouring a primary N. brasiliensis infection. The proportion of this radioactivity which was incorporated into soluble mucin was also increased, to reach a peak 13 days after infection when the worms were being expelled. A new model of infection was also developed, whereby expulsion of immature adult worms occurred within 4 hours of intraduodenal challenge. Macroscopic and microscopic examination of the intestine indicated that mucus may serve both as a barrier to and a trapping agent of N. brasiliensis in immune rats. This is in agreement with similar observations made by Lee and Ogilvie, 1980, for Trichinella spiralis infection in the rat.
Part of this work was done while H.R.P. Miller was a member of the Department of Immunology, John Curtin School, Canberra, Australia.
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© 1981 ECSC, EEC, EAEC, Brussels-Luxembourg
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Miller, H.R.P., Huntley, J.F., Dawson, A.M. (1981). Mucus Secretion in the Gut, Its Relationship to the Immune Response in Nippostrongylus-Infected Rats. In: Bourne, F.J. (eds) The Mucosal Immune System. Current Topics in Veterinary Medicine and Animal Science, vol 12. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-8331-1_20
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