RNA Gel Electrophoresis: A Technique to Monitor Laboratory Cross-Contaminations and Genetic Reassortment Amongst Rotavirus Isolates

  • S. Tzipori
  • M. Smith
Part of the Current Topics in Veterinary Medicine and Animal Science book series (CTVM, volume 13)

Abstract

The RNA of 2 isolates of simian rotavirus SAll and another cell culture adapted isolate 325, are compared using Polyacrylamide slab gel electrophoresis. The 2 SAH isolates differ in the positions of RNA segments 3, 325 is believed to exist as a mixed population on the basis of RNA analysis and the RNA pattern of the predominant viral element is indistinguishable from that of one of the SAll isolates. It is suggested that genetic reassortment of the RNA segments in mixed infections can produce a progeny rotavirus with an altered genome.

Keywords

Migration Electrophoresis Trypsin Polyacrylamide ECSC 

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Copyright information

© ECSC, EEC, EAEC, Brussels-Luxembourg 1981

Authors and Affiliations

  • S. Tzipori
    • 1
  • M. Smith
    • 1
  1. 1.Attwood Veterinary Research LaboratoryWestmeadows, MelbourneAustralia

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