RNA Gel Electrophoresis: A Technique to Monitor Laboratory Cross-Contaminations and Genetic Reassortment Amongst Rotavirus Isolates
The RNA of 2 isolates of simian rotavirus SAll and another cell culture adapted isolate 325, are compared using Polyacrylamide slab gel electrophoresis. The 2 SAH isolates differ in the positions of RNA segments 3, 325 is believed to exist as a mixed population on the basis of RNA analysis and the RNA pattern of the predominant viral element is indistinguishable from that of one of the SAll isolates. It is suggested that genetic reassortment of the RNA segments in mixed infections can produce a progeny rotavirus with an altered genome.
KeywordsMigration Electrophoresis Trypsin Polyacrylamide ECSC
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