Abstract
A direct microplate ELISA for the detection and quantitation of bovine rotavirus is described. The assay permitted the detection of less than 5 ng virus/ml in purified preparations and in faecal samples, providing that the samples contained enough virus so that the assay could be performed at dilutions ⩾ 1:1000. In spite of a reduction in the sensitivity of the test at high concentrations of faecal material, the direct assay proved, in practice, to be more efficient than was electron microscopy. Results on the excretion of rotavirus in the course of infections and on the incidence of rotavirus infections among calves and older animals are presented and discussed.
A variant procedure that permits the detection of rotavirus immune complexes is described. Preliminar results are presented indicating that this test may constitute a useful tool in diagnostic work and in immunological investigations.
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© 1981 ECSC, EEC, EAEC, Brussels-Luxembourg
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Scherrer, R., Corthier, G., L’Haridon, R. (1981). ELISA for the Detection of Rotavirus and Rotavirus/Antibody Complexes in Faeces. In: de Leeuw, P.W., Guinée, P.A.M. (eds) Laboratory Diagnosis in Neonatal Calf and Pig Diarrhoea. Current Topics in Veterinary Medicine and Animal Science, vol 13. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-8328-1_5
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DOI: https://doi.org/10.1007/978-94-009-8328-1_5
Publisher Name: Springer, Dordrecht
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