Abstract
A polystyrene solid phase is coated with antibody directed against the K88 antigen of E. coli. During incubation of the coated surface with a faecal extract, antigen (if present) will be caught by specific antibody. This antigen can be labeled with antibody covalently linked to the enzyme horseradish peroxidase (conjugate). The bound conjugate can be visualized by the addition of a chromogenic substrate.
Laboratory manual of the Central Veterinary Institute, Immunology Department, 3002 AA Rotterdam, The Netherlands
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© 1981 ECSC, EEC, EAEC, Brussels-Luxembourg
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de Leeuw, P.W., Guinée, P.A.M. (1981). Enzyme-Linked Immunosorbent Assay (Elisa) for the Detection of the K88 Antigen of Escherichia Coli in Porcine Faeces. In: de Leeuw, P.W., Guinée, P.A.M. (eds) Laboratory Diagnosis in Neonatal Calf and Pig Diarrhoea. Current Topics in Veterinary Medicine and Animal Science, vol 13. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-8328-1_22
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DOI: https://doi.org/10.1007/978-94-009-8328-1_22
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-009-8330-4
Online ISBN: 978-94-009-8328-1
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