Abstract
The current techniques available for the detection of transmissible gastroenteritis virus, bovine and porcine rotaviruses, bovine enteric Coronavirus, bovine astrovirus and bovine parvovirus are reviewed. Although, initially, the enteric pathogens were difficult to establish in culture regularly, cell culture methods are currently available for identification of several of them. Some of the viruses can be detected by serial passage with cytopathology but many techniques rely on the production of antigens which are identified by immunofluorescent staining. The techniques which have been used to detect serological differences between the viruses and to detect viral antibody are reviewed and some of the advantages and disadvantages of cell culture systems are discussed.
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© 1981 ECSC, EEC, EAEC, Brussels-Luxembourg
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Bridger, J.C. (1981). Cell Culture Techniques for the Identification of Enteric Viruses — Present Possibilities. In: de Leeuw, P.W., Guinée, P.A.M. (eds) Laboratory Diagnosis in Neonatal Calf and Pig Diarrhoea. Current Topics in Veterinary Medicine and Animal Science, vol 13. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-8328-1_2
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DOI: https://doi.org/10.1007/978-94-009-8328-1_2
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