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Laboratory manual of the Central Veterinary Institute, Virology Department, 8221 RA Lelystad, The Netherlands

  • P. W. de Leeuw
  • P. A. M. Guinée
Part of the Current Topics in Veterinary Medicine and Animal Science book series (CTVM, volume 13)

Abstract

A polystyrene solid phase is coated with antibodies directed against bovine-rotavirus antigens. Upon addition of a faecal extract, rotavirus antigens (if present) will be trapped by specific antibodies covalently linked to the enzyme peroxidase (conjugate). The bound conjugate is visualized through addition of a chromogenic substrate. Specificity of the reaction is checked by blocking the conjugate reaction with a specific anti-rotavirus serum.

Keywords

Disodium Hydrogen Phosphate Faecal Extract ELISA Unit Reference Seron Haemagglutinating Activity 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Ellens, D.J. and P.W. de Leeuw: Enzyme-linked immunosorbent assay for diagnosis of rotavirus infections in calves. J. clin. Microbiol. 6, 530–532, 1977.PubMedGoogle Scholar
  2. J.A.M. van Balken, P.W. de Leeuw, D.J. Ellens and P.J. Strayer: Detection of coronavirus in calf faeces with a haemadsorption — elution — haemagglutination assay (HEHA). Vet. Microbiol. 3, 205–211, 1978/1979.CrossRefGoogle Scholar

Copyright information

© ECSC, EEC, EAEC, Brussels-Luxembourg 1981

Authors and Affiliations

  • P. W. de Leeuw
    • 1
  • P. A. M. Guinée
    • 2
  1. 1.Centraal Diergeneeskundig InstituutLelystadThe Netherlands
  2. 2.Rijksinstituut voor de VolksgezondheidBilthovenThe Netherlands

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