Abstract
A polystyrene solid phase is coated with antibodies directed against bovine-rotavirus antigens. Upon addition of a faecal extract, rotavirus antigens (if present) will be trapped by specific antibodies covalently linked to the enzyme peroxidase (conjugate). The bound conjugate is visualized through addition of a chromogenic substrate. Specificity of the reaction is checked by blocking the conjugate reaction with a specific anti-rotavirus serum.
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Ellens, D.J. and P.W. de Leeuw: Enzyme-linked immunosorbent assay for diagnosis of rotavirus infections in calves. J. clin. Microbiol. 6, 530–532, 1977.
J.A.M. van Balken, P.W. de Leeuw, D.J. Ellens and P.J. Strayer: Detection of coronavirus in calf faeces with a haemadsorption — elution — haemagglutination assay (HEHA). Vet. Microbiol. 3, 205–211, 1978/1979.
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© 1981 ECSC, EEC, EAEC, Brussels-Luxembourg
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de Leeuw, P.W., Guinée, P.A.M. (1981). Laboratory manual of the Central Veterinary Institute, Virology Department, 8221 RA Lelystad, The Netherlands. In: de Leeuw, P.W., Guinée, P.A.M. (eds) Laboratory Diagnosis in Neonatal Calf and Pig Diarrhoea. Current Topics in Veterinary Medicine and Animal Science, vol 13. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-8328-1_13
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DOI: https://doi.org/10.1007/978-94-009-8328-1_13
Publisher Name: Springer, Dordrecht
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