Laboratory manual of the Central Veterinary Institute, Virology Department, 8221 RA Lelystad, The Netherlands

  • P. W. de Leeuw
  • P. A. M. Guinée
Part of the Current Topics in Veterinary Medicine and Animal Science book series (CTVM, volume 13)


A polystyrene solid phase is coated with antibodies directed against bovine-rotavirus antigens. Upon addition of a faecal extract, rotavirus antigens (if present) will be trapped by specific antibodies covalently linked to the enzyme peroxidase (conjugate). The bound conjugate is visualized through addition of a chromogenic substrate. Specificity of the reaction is checked by blocking the conjugate reaction with a specific anti-rotavirus serum.


Cellulose Hydrogen Peroxide Glycerol Filtration Albumin 


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  1. Ellens, D.J. and P.W. de Leeuw: Enzyme-linked immunosorbent assay for diagnosis of rotavirus infections in calves. J. clin. Microbiol. 6, 530–532, 1977.PubMedGoogle Scholar
  2. J.A.M. van Balken, P.W. de Leeuw, D.J. Ellens and P.J. Strayer: Detection of coronavirus in calf faeces with a haemadsorption — elution — haemagglutination assay (HEHA). Vet. Microbiol. 3, 205–211, 1978/1979.CrossRefGoogle Scholar

Copyright information

© ECSC, EEC, EAEC, Brussels-Luxembourg 1981

Authors and Affiliations

  • P. W. de Leeuw
    • 1
  • P. A. M. Guinée
    • 2
  1. 1.Centraal Diergeneeskundig InstituutLelystadThe Netherlands
  2. 2.Rijksinstituut voor de VolksgezondheidBilthovenThe Netherlands

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