Abstract
The purpose of this study was to investigate changes in the number of NoRs in successive zones (1st, 3rd, 5th and 7th mm segments from the root tip) of growth and differentiation of roots in 4 plant species characterized by the presence or absence of endopolyploid nuclei. Cytophotometric measurements of the DNA content (after Feulgen’s procedure, hydrolysis for 1 h in 4N HC1 at 20 °C) have shown that in supra-meristematic zones (3rd, 5th and 7th segments) in Haemanthus katharinae and Tulipa kaufmanniana all nuclei have 2C DNA content (Figs. 3A and 4A), in Zea mays 4C and 8C nuclei are present, while in Helianthus annuus in the 5th and 7th segments some nuclei reveal 4C and 8C DNA content (comp. [8]).
This work was supported by the Polish Academy of Sciences within the project 09.7.1.5.4.
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References
Avanzi, S., Durante, M., Cionini, G. and D’Amato, F. 1972 Cytological localization of ribosomal cistrons in polytene chromosomes of Phaseolus coccineus. Chromosoma (Berl.) 39, 191–203.
Giménez-Martin, G., de la Torre, C., Lopez-Saez, J. F. and Esponda, P. 1977 Plant nucleolus: structure and physiology. Cytobiologie 14, 421–462.
Hernandez-Verdun, D., Hubert, J., Bourgeois, C. A. and Bouteille, M. 1980 Ultrastructural localization of Ag-NoR stained proteins in the nucleolus during the cell cycle and in other nucleolar structures. Chromosoma (Berl.) 79, 349–362.
Maggini, F., De Dominicis, R. I. and Salvi, G. 1976 Similarities among ribosomal RNA’s of Angiospermae and Gymnospermae. J. Mol. Evol. 8, 329–335.
Kononowicz, A. K., Kuran, H. and Olszewska, M. J. 1976 Cytochemical and ultrastructural studies of changes in the nucleus and nucleolus during cell differentiation in the root cortex of Haemanthus katharinae. Folia Histochem. Cytochem. 14, 151–164.
Olszewska, M.J. 1976 Autoradiographic and ultrastructural study of Cucurbita pepo root cells during their growth and differentiation. Histochemistry 49, 157–175.
Olszewska, M. J. and Kononowicz, A. K. 1979 Activities of DNA polymerases and RNA polymerases detected in situ in growing and differentiating cells of root cortex. Histochemistry 59,311–323.
Olszewska, M. J. and Kononowicz, A. K. 1981 Can induced autopolyploidy replace naturally occurring endopolyploidization in roots? Proc. 2nd Symp. on Structure and Function of Roots, this volume, pp. 49–51.
Schubert, I., Anastassova-Kristeva, M. and Rieger, R. 1979 Specificity of NoR staining in Vicia faba. Exp. Cell Res. 120, 433–435.
Spear, B. 1974 The genes for ribosomal RNA in diploid and polytene chromosomes of Drosophila melanogaster. Chromosoma (Berl.) 48, 159–179.
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© 1981 Martinus Nijhoff/Dr W. Junk Publishers, The Hague/Boston/London
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Olszewska, M.J., Kononowicz, A.K., Kononowicz, H., Janas, K. (1981). In situ 3H rRNA/DNA hybridization and silver staining of NORs during growth and differentiation of root cortex cells in the presence or absence of DNA endoreplication. In: Brouwer, R., Gašparíková, O., Kolek, J., Loughman, B.C. (eds) Structure and Function of Plant Roots. Developments in Plant and Soil Sciences, vol 4. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-8314-4_10
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DOI: https://doi.org/10.1007/978-94-009-8314-4_10
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