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Orotic Acid pp 14-17 | Cite as

Aggregation of Enzymes of the Orotate Pathway

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Abstract

During purification and heat denaturation experiments the activity of orotidylic acid decarboxylase appears to parallel orotate phosphoribosyltransferase activity. Also under a variety of physiological conditions both enzymes show closely parallel, co-ordinate changes in activity [120–122]. Orotate phosphoribosyltransferase from murine leukaemia P 1534 J (utilizing as the substrate orotate and 5-fluorouracil) was found to exist as a complex with orotidylic acid decarboxylase (123). Both enzyme activities were eluted together during gel filtration, co-sedimented in sucrose gradients, and remainded associated during salt fractionation. However, they could be separated into a phosphoribosyltransferase and decarboxylase component when enzyme preparations previously subjected to limited proteolysis by elastase were sedimented in sucrose gradients F[123].

Keywords

Sucrose Gradient Limited Proteolysis Ehrlich Ascites Carcinoma OROTIC Acid Carbamoyl Phosphate Synthetase 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© A. Čihák and W. Reutter 1980

Authors and Affiliations

  1. 1.Institute of Organic Chemistry and BiochemistryCzechoslovakia Academy of Sciences PragueCzechoslovakia
  2. 2.Institut für Molekularbiologie und Biochemie der Freien UniversitätBerlinGermany

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