Orotic Acid pp 14-17 | Cite as

Aggregation of Enzymes of the Orotate Pathway



During purification and heat denaturation experiments the activity of orotidylic acid decarboxylase appears to parallel orotate phosphoribosyltransferase activity. Also under a variety of physiological conditions both enzymes show closely parallel, co-ordinate changes in activity [120–122]. Orotate phosphoribosyltransferase from murine leukaemia P 1534 J (utilizing as the substrate orotate and 5-fluorouracil) was found to exist as a complex with orotidylic acid decarboxylase (123). Both enzyme activities were eluted together during gel filtration, co-sedimented in sucrose gradients, and remainded associated during salt fractionation. However, they could be separated into a phosphoribosyltransferase and decarboxylase component when enzyme preparations previously subjected to limited proteolysis by elastase were sedimented in sucrose gradients F[123].


Sucrose Gradient Limited Proteolysis Ehrlich Ascites Carcinoma OROTIC Acid Carbamoyl Phosphate Synthetase 
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Copyright information

© A. Čihák and W. Reutter 1980

Authors and Affiliations

  1. 1.Institute of Organic Chemistry and BiochemistryCzechoslovakia Academy of Sciences PragueCzechoslovakia
  2. 2.Institut für Molekularbiologie und Biochemie der Freien UniversitätBerlinGermany

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