Aspects of aerobic mineralization during spring in Lake Vechten with special reference to the 14C-labelling technique

  • J. J. Olie
  • Th. E. Cappenberg
Part of the Developments in Hydrobiology book series (DIHY, volume 11)

Abstract

Aerobic mineralization, i.e. seston respiration, microbial breakdown of detritus and microbial assimilation-dissimilation of photosynthetically derived D(issolved) O(rganic) C(arbon) was measured in concentrated samples from the pelagic zone of Lake Vechten. The samples were described by cell numbers of dominant algae prior to concentrating by centrifuge with continuous rotor. The concentrated samples were incubated in the laboratory at in situ temperature and a light intensity of 30 W · m-2 for measuring primary production and photosynthetically derived DOC. After filtration the particulate fraction was incubated in unlabelled lake water for measuring respiratory production of DI14C. Portions of the same particulate fraction were sterilized and incubated in unlabelled sample concentrate for measuring microbial breakdown of detritus. The seston respiration amounted to 19–30% loss of the particulate fraction over 15–45 h. Microbial breakdown of detritus amounted to 28–40% loss of the particulate fraction over 24–168 h. In both cases P(articulate) O(rganic) 14C(arbon) was transformed to mainly DI14C. Microbial assimilation-dissimilatioh of photosynthetically derived DOC could not be measured reliably.

Keywords

aerobic mineralization respiration photo-assimilation extracellular release carbon recycling 

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References

  1. Bell, W. H. & Sakshaug, E., 1980. Bacterial utilization of algal extracellular products. 2. A kinetic study of natural populations. Limnol. Oceanogr. 25: 1021–1033.CrossRefGoogle Scholar
  2. Berman, T. & Gerber, C, 1980. Differential filtration studies of carbon flux from living algae to microheterotrophs, microplankton size distribution and respiration in Lake Kinneret. Microb. Ecol. 6: 189–198.CrossRefGoogle Scholar
  3. Blaauboer, M. A. C. I., 1982. The phytoplankton species composition and the seasonal periodicity in Lake Vechten from 1956 to 1979. Hydrobiologia 95: 25–36.CrossRefGoogle Scholar
  4. Blaauboer, M. A. C. I., van Keulen, R. & Cappenberg, Th. E. (in press). Extracellular release of photosynthetic products by freshwater phytoplankton populations, with special reference to the algal species involved. Freshwat. Biol.Google Scholar
  5. Cappenberg, Th. E., 1976. Methanogenesis in the bottom deposits of a small stratifying lake. In: ( Schlegel, H. G., Pfennig, N., Gottschalk, G., (Eds.). Microbial Production and Utilization of Gases, H2, CH4, CO, E. Goltze-Verlag, Göttingen, pp. 125–134.Google Scholar
  6. Cappenberg, Th. E. & Verdouw, H., 1982. Sedimentation and breakdown kinetics of organic matter in the anaerobic zone of Lake Vechten. Hydrobiologia 95: 165–179.CrossRefGoogle Scholar
  7. Cole, J. J. & Likens, G. E., 1979. Measurements of mineralization of phytoplankton detritus in an oligotrophic lake. Limnol. Oceanogr. 24: 541–547.CrossRefGoogle Scholar
  8. Derenbach, J. B. & Williams, P. J., 1974. Autotrophic and bacterial production: fractionation of plankton populations by differential filtration of samples from the English Channel. Mar. Biol. 25: 263–269.CrossRefGoogle Scholar
  9. Eppley, R. W. & Sharp, J. H., 1975. Photosynthetic measurements in the Central North Pacific: the dark loss of carbon in 24 h incubations. Limnol. Oceanogr. 20: 981–987.CrossRefGoogle Scholar
  10. Golterman, H. L., Clymo, R. S. & Ohnstad, M. A. M., 1978. Methods for Physical and Chemical Analysis of Fresh Waters. IBP Handbook No. 8. Blackwell Scientific, Oxford.Google Scholar
  11. Gulati, R. D., 1977. Vertical changes in the filtering, feeding and assimilation rates of dominant zooplankters in a stratified lake. Verh. int. Verein. Limnol. 20: 950–956.Google Scholar
  12. Herbland, A., 1975. Utilisation par la flore héterotrophe de la matière organique naturelle dans l’eau de mer. J. exp. mar. Biol. Ecol. 19: 19–31.CrossRefGoogle Scholar
  13. Iturriaga, R. & Hoppe, H. G., 1977. Observations of heterotrophic activity on photoassimilated organic matter. Mar. Biol. 40: 101–108.CrossRefGoogle Scholar
  14. Steenbergen, C. L. M. & Korthals, H. J. (in press). Distribution of phototrophic microorganisms in the anaerobic and microaerophilic strata of Lake Vechten. Pigments analysis and role in primary production. Limnol. Oceanogr.Google Scholar
  15. Steenbergen, C. L. M. & Verdouw, H., 1982. Lake Vechten: aspects of its morphometry, climate, hydrology and physicochemical characteristics. Hydrobiologia 95: 11–23.CrossRefGoogle Scholar
  16. Utermöhl, U., 1958. Zur Vervollkommnung der quantitativen Phytoplankton methodik. Mitt: int. Verein. Limnol. 9: 1–38.Google Scholar
  17. Wiebe, W. J. & Smith, D. F., 1977a. Direct measurement of dissolved organic carbon release by phytoplankton and incorporation by microheterotrophs. Mar. Biol. 42: 213–223.CrossRefGoogle Scholar
  18. Wiebe, W. J. & Smith, D. F., 1977b. 14C-labelling of the compounds excreted by phytoplankton for employment as a realistic tracer in secondary productivity measurements. Microb. Ecol. 4: 1–8.Google Scholar

Copyright information

© Dr W. Junk Publishers, The Hague 1982

Authors and Affiliations

  • J. J. Olie
    • 1
  • Th. E. Cappenberg
    • 1
  1. 1.‘Vijverhof’ LaboratoryLimnological InstituteNieuwersluisThe Netherlands

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