The Sperm Cell pp 328-331 | Cite as

Human Sperm Velocities, Evaluation by Microcinematography and Laser Doppler Velocimetry (LDV)

  • P. Jouannet
  • C. Serres
  • D. Feneux
  • C. Jeulin
  • G. David

Abstract

An efficient motility of sperm is necessary for their penetration into the cervical mucus, transport through the female genital ducts and crossing of the periovular layers (1). Assessments of sperm motility are most of the time approximative and subjective. In seminal plasma, it is currently thought that the “good” spermatozoa have to swim straight and fast; in the vicinity of the ovocyte, the “whiplash” type of movement is often described as characteristic of the activated spermatozoa. The velocity is a motility parameter which can be measured by various techniques (2) but there is not a general agreement on the type of velocity measured and its signification. We describe the different kinds of velocities which may be measured by microcinematography (MC) and laser Doppler Velocimetry (LDV) in seminal plasma (SP) and cervical mucus (CM). The spermatozoa were filmed at 20–22° C according to a technique previously described (3). For studies in SP, 25 μl of semen samples were placed between a slide and a 22 × 32 coverslip (approximative depth: 30μm). For studies in CM, cine was proceeded one hour after in vitro penetration of spermatozoa in preovulatory CM gently aspirated in a 1 mm square glass pipette (4). LDV is a technique analysing the light frequency changes induced by and related to sperm velocities according to Doppler effect (2). The experimental conditions for LDV measurements were the same as those used for MC except the depth of the studied semen sample (100 μm).

Keywords

Velocimetry 

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References

  1. Katz, D.F., Overstreet J.W., 1980: Testicular development, structure, and function, pp. 481–489, ed. A. Steinberger. Raven Press, New YorkGoogle Scholar
  2. Dubois, M., Jouannet P., Berge, P., Volochine, B., Serres, C., David, G., 1975, Ann. Phys. Biol. Med., 9, 19–14.Google Scholar
  3. David, G., Serres, C., Juoannet, P., 1981, Gamete Research, 4, 83–95CrossRefGoogle Scholar
  4. Dubois, M., Jouannet, P., Berger, P., David, G., 1982, Nature 252, 711–713CrossRefGoogle Scholar
  5. Serres C., Feneux D., Juoannet P., David G. 1982, (this meeting)Google Scholar
  6. Prost J., Cummins H.Z., Pritchett T.R. 1981, Science 212, 1520–1522CrossRefGoogle Scholar
  7. Katz D.F. Mills R.N. Pritcett T.R. 1978, J. Reprod. Fert. 53, 259–265CrossRefGoogle Scholar
  8. Katz D.F. Overstreet J. W. Hanson F.W. 1981, J. Reprod. Fert. 62, 221–228Google Scholar
  9. Makler A., Itskovitz J., Brandes J.M. Paldi E. 1979, Fertil. Steril, 31, 155–161PubMedGoogle Scholar

Copyright information

© Martinus Nijhoff Publishers, The Hague 1983

Authors and Affiliations

  • P. Jouannet
    • 1
  • C. Serres
    • 1
  • D. Feneux
    • 1
  • C. Jeulin
    • 1
  • G. David
    • 1
  1. 1.Centre HospitalierKremlin BicetreFrance

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