Is Sperm α-L-Fucosidase Responsible for Sperm-Egg Binding in Ciona Intestinalis?
Species-specific and self-non self binding of spermatozoa to the vitelline coat is an obligatory step for fertilization in Ciona intestinalis (1,2). It is known that terminal fucose residues of the glycoproteins of the outer surface of the vitelline coat play the major role in the binding (3,4). However, it is yet to be proved that the counterpart on the sperm surface is a fucose-binding protein. This paper presents evidence that α-L-fucosidase on the sperm surface is responsible for the binding.
KeywordsAmmonium Sulphate Ammonium Sulphate Precipitation Sperm Surface Active Sperm Glycosidase Activity
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