Abstract
Doxorubicin is one of our most widely used and potent drugs against human cancer. It is generally assumed that doxorubicin has to enter the cell across the plasma membrane in order to exert its therapeutic effect by interference with the function of the DNA in the cell nucleus through intercalation (1). The transport mechanism by which doxorubicin enters the cell across the rate-limiting barrier is still under debate. It has been proposed that doxorubicin is actively extruded from the cells, counteracting a facilitated diffusion transport process (a carrier-mediated transport) into the cells because 1) the cellular doxorubicin uptake increases in the presence of metabolic inhibitors, and 2) the doxorubicin transport shows saturation kinetics, self-inhibition, and substrate competition (2–4). However, a membrane-bound doxorubicin-activated ATP’ase has not yet been demonstrated. So far, doxorubicin has been demonstrated to be an inhibitor of the Na,K-activated membrane-bound ATP’ase (5). Furthermore, in order to understand the transmembraneous transport mechanism of a compound, one must demonstrate how the physicochemical characteristics of the compound in the water phases on both sides of the membrane are affected, for example, by the concentration of the compound itself, the concentration of other compounds, the temperature, and the pH. The purpose of the present paper is 1) to summarize data on some of the physicochemical properties of doxorubicin in aqueous solution, and 2) to describe how these properties apparently explain some of the features of doxorubicin transport across biological membranes.
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References
Pigram, WJ, Fuller W, Hamilton LD. 1972. Stereochemistry of intercalation: Interaction of daunanycin with DNA. Nature (London), New Biol. 235: 17–19.
Danoe KJ. 1976. Experimentally developed resistance to daunanycin. Acta Path. Microbiol. Scand., suppl. 256.
Skovsgaard T. 1977. Transport and binding of daunanycin, adriamycin and rubidazone in Ehrlich ascites tumour cells. Biochem. Pharmacol. 26: 215–222.
Skovsgaard T. 1978. Carrier-mediated transport of daunanycin, adriamycin and rubidazone in Ehrlich ascites tumor cells. Biochem. Pharmacol. 27: 1221–1227.
Gosalves M, van Rossum GDV, Blanco MF. 1979. Inhibition of sodiumpotassium-activated adenosine 5#x2019;-triphosphatase and ion transport by adriamycin. Cancer Res. 39: 257–261.
Arcamone F, Cassinelli G, Franceschi G, Penco S, Pol C, Redaelli S, Selva A. 1972. Structure and physicochemical properties of adriamycin (doxorubicin). In: Int. Symp. Adriamycin (Carter SK, Di Marco A, Ghione M, Krakoff IH, Mathe G, eds.). p;New York, Springer-Verlag, pp. 9–22.
Bates RG. 1973. Determination of pH. 2nd ed. New York, John Wiley, p. 90.
Dalmark M, Storm HH. In press. A Fickian diffusion transport process with features of transport catalysis: Doxorubicin transport in human red blood cells. J. Gen. Physiol.
Barthelemy-Clavey V, Maurizot J-C, Dimicoli J-L, Sicard P. 1974. Self-association of daunomycin. FEBS Lett. 46: 5–10.
Eksborg S. 1978. Extraction of daunanycin and doxorubicin and their hydroxyl metabolites: Self-association in aqueous solutions. J. Pharm. Sci. 67: 782–785.
Righetti PG, Menozzi M, Gianazza E, Valentini L. 1979. Proto lytic equilibria of doxorubicin as determined by isoelectric focusing and “electrophoretic titration curves.#x201D; FEBS Lett. 101: 51–55.
Bachur NR, Moore AL, Bernstein JG, Liu A. 1970. Tissue distribut~on and disposition of daunomycin in mice: Fluoranetric and isotopic methods. Cancer Chemother. Rep. 54: 89–94.
Duarte-Karim M, Ruysschaert JM, Hildebrand J. 1976. Affinity of adriamycin to phospholipids: A possible explanation for cardiac mitochondrial lesions. Biochem. Biophys. Res. Commun. 71: 658–663.
Goormaghtigh E, Chatelain P, Caspers J, Ruysschaert JM. 1980. Evidence of a specific complex between Adriamycin and negatively charged phosphlipids. Biochim. Biophys. Acta 597: 1–14.
Goormagtigh E, Chatelain P, Caspers J, Ruysschaert JM. 1980. Evidence of a complex between Adriamycin derivatives and cardiolipin: Possible role in cardiotoxicity. Biochem. Pharmacol. 29: 3003–3010.
Menozzi M, Arcamone F. 1978. Binding of adriamycin to sulphated mucopolysaccharides. Biochem. Biophys. Res. Commun. 80: 313–318.
Kikuchi H, Sato S. 1976. Binding of daunomycin to nonhistone proteins from rat liver. Biochim. Biophys. Acta 434: 509–512.
Chao Na, Tirnasheff SN. 1977. Physical-chemical study of daunornycintubu1in interactions. Arch. Biochem. Biophys. 182: 147–154.
Kharasch ED, Novak RF. 1980. Ring current effect in Adriamycin-flavin mononucleotide complexation as observed by H FT NMR spectroscopy. Biochem. Biophys. Res. Commun. 92: 1320–1326.
Brock N, Druckrey H, Hamperl H. 1938. Zur Wirkungsweise carcinogener Substanzen. Arch. Exp. Pathol. Pharmakol. 189: 709–731.
Dalrnark M, Johansen P. 1981. Regulations of doxorubicin (Adriamycin) transport across biological membranes by complex formation with nucleotides, nucleosides, and DNA-derived bases. Proc. Am. Assoc. Cancer Res. 22: 31.
Dalrnark M. In press. Characteristics of doxorubicin transport in human red blood cells. Scand. J. C1in. Lab. Invest.
Dalrnark M, Wieth JO. 1972. Temperature dependence of chloride, bromide, iodide, thiocyanate and salicylate transport in human red cells. J. Physiol. (London) 224: 583–610.
Mikkelsen RD, Peck-Sun L, Wallach DFH. 1977. Interaction of adridffiycin with human red blood cells: A biochemical and morphological study. J. Mol. Med. 2: 33–40.
Tritton TR, Murphree SA, Sartorelli AC. 1978. Citation in Biochim. Biophys. Acta 512: 254–269. (See ref. 26.)
Goldman R, Facchinatti T, Bach D, Raz A, Shinitzky M. 1978. A differential interaction of daunomycin, adriamycin and their derivatives with human erythrocytes and phospholipids bilayers. Biochim. Biophys. Acta 512: 254–269.
Kessel D. 1979. Biologic properties of three anthracyc1ines as a function of lipophi1icity. Biochem. Pharmacol. 28: 3028–3030.
Egorin MJ, Clawson RE, Ross LA, Bachur NR. 1980. Cellular pharmacology of N,N-dimethyl daunomycin and N,N-dimethyl Adriarnycin. Cancer Res. 40: 1928–1933.
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© 1982 Martinus Nijhoff Publishers, The Hague
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Dalmark, M. (1982). The Physicochemical Properties and Transmembraneous Transport of Doxorubicin. In: Muggia, F.M., Young, C.W., Carter, S.K. (eds) Anthracycline Antibiotics in Cancer Therapy. Developments in Oncology, vol 10. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-7630-6_15
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DOI: https://doi.org/10.1007/978-94-009-7630-6_15
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