Complement—Dependent Neutralisation of Aujeszky’s Disease Virus by Antibody

  • V. Bitsch
  • M. Eskildsen
Part of the Current Topics in Veterinary Medicine and Animal Science book series (CTVM, volume 17)

Abstract

The complement-dependent neutralisation reaction in tests for demonstration of Aujeszky’s disease virus-neutralising antibody (VNA) was studied. Guinea pig serum was the source of complement (C′) and the concentrations giving optimum augmentation of antibody titres were determined. Both IgM and IgG contained C′-requiring VNA. IgM antibody produced early in the course of immunisation showed the highest degree of C′-dependency, while no difference in the respect could be found between early and late IgM antibody. Appropriate concentrations of C′ enhanced IgM antibody titres by up to 9 log2 units and raised IgG antibody titres 3 to 4 log2 units. A linear relationship was demonstrated between C′-enhanced VNA titres and the pre-incubation period. C′ was found to act only on performed infectious virus-antibody complexes, and the full effect could be achieved by adding C′ to the virus-serum mixtures a relatively short time before inoculation of cell cultures. Addition of C′ to the virus-serum mixtures at the beginning of preincubation gave reduced enhancement of VNA titres.

Keywords

Titration Sera ECSC Rhinotracheitis 

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References

  1. Ashe, W.K. and Notkins, A.L., 1966. Neutralization of an infectious herpes simplex virus-antibody complex by anti-y-globulin. Proc. Nat. Acid. Sci., 56, 447–451.CrossRefGoogle Scholar
  2. Bitsch, V., 1978. An investigation into the basic virus-antibody neutralization reaction, with special regard to the reaction in the constant- virus /varying -serum neutralization test. Acta. Vet. Scand., 19, 110–128.PubMedGoogle Scholar
  3. Curtis, J. and Bourne, F.J., 1971. Immunoglobulin quantitation in sow serum, colostrum and milk and the serum of young pigs. Biochem. Biophys. Acta (Amst.), 236, 319–332.Google Scholar
  4. Eskildsen, M., 1975. Demonstration of antibodies against pseudorabies virus in swine serum by a modified direct complement fixation test. Bull. Off. Int. Epiz., 84, 253–263.Google Scholar
  5. Metzger, J.J. and Fougereau, M., 1967. Caracterisation de deux sous-classes d’immunoglobulines γ G chez le pore. C.R. Acad. Sci. (Paris), 265, 724–727.Google Scholar
  6. Rossi, C.R. and Kiesel, G.K., 1976. Antibody class and complement requirement of neutralizing antibodies in the primary and secondary antibody response of cattle to infectious bovine rhinotracheitis virus vaccine. Arch. Virology, 51, 191–198.CrossRefGoogle Scholar
  7. Taniguchi, S. and Yoshino, K., 1965. Studies on the neutralization of herpes simplex virus. II Analysis of complement as the antibody- potentiating factor. Virology, 26, 54–60.PubMedCrossRefGoogle Scholar
  8. Yoshino, K. and Taniguchi, S., 1964. The appearance of complement-requiring neutralizing antibodies by immunization and infection with herpes simplex virus. Virology, 22, 193–201.PubMedCrossRefGoogle Scholar

Copyright information

© ECSC, EEC, EAEC, Brussels-Luxemburg 1982

Authors and Affiliations

  • V. Bitsch
    • 1
  • M. Eskildsen
    • 2
  1. 1.State Veterinary Serum LaboratoryCopenhagen VDenmark
  2. 2.State Veterinary Institute for Virus ResearchLindholm, KalvehaveDenmark

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