Complement—Dependent Neutralisation of Aujeszky’s Disease Virus by Antibody
The complement-dependent neutralisation reaction in tests for demonstration of Aujeszky’s disease virus-neutralising antibody (VNA) was studied. Guinea pig serum was the source of complement (C′) and the concentrations giving optimum augmentation of antibody titres were determined. Both IgM and IgG contained C′-requiring VNA. IgM antibody produced early in the course of immunisation showed the highest degree of C′-dependency, while no difference in the respect could be found between early and late IgM antibody. Appropriate concentrations of C′ enhanced IgM antibody titres by up to 9 log2 units and raised IgG antibody titres 3 to 4 log2 units. A linear relationship was demonstrated between C′-enhanced VNA titres and the pre-incubation period. C′ was found to act only on performed infectious virus-antibody complexes, and the full effect could be achieved by adding C′ to the virus-serum mixtures a relatively short time before inoculation of cell cultures. Addition of C′ to the virus-serum mixtures at the beginning of preincubation gave reduced enhancement of VNA titres.
KeywordsTitration Sera ECSC Rhinotracheitis
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