Abstract
The early difficulties and failures to grow hepatitis viruses in tissue culture or to infect convenient and available laboratory animals emphasise the necessity for reliable immunochemical, biophysical or biochemical procedures for diagnosing the acute diseases, for detecting the presence of the viruses in biological specimens and fluids, and for serologic analyses for antibodies in serum as evidences of exposure. Viruses in serum are usually not detectable by immunologic procedures during the viraemic phase of most diseases. Diagnosis of most viral diseases is usually achieved by isolating the cytopathic agent in tissue culture from clinical specimens, or by infecting susceptible animals with specimens believed to contain the virus. Alternately, an increase in antibody titre or seroconversion during acute disease and convalescence can be diagnostic. The presence of antibody can be observed by neutralization of the infectious agent, or by immunochemical reactions with virus antigens.
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© 1982 Martinus Nijhoff Publishers, The Hague
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Overby, L.V. (1982). Serodiagnosis and Control in Viral Hepatitis. In: Sibinga, C.T.S., Das, P.C., Forfar, J.O. (eds) Paediatrics and Blood Transfusion. Developments in Hematology and Immunology, vol 2. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-7520-0_27
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DOI: https://doi.org/10.1007/978-94-009-7520-0_27
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