In vitro quantitative assay of invasion using human amnion
The basic mechanisms by which tumor cells invade host tissue are poorly understood largely because this process has been difficult to study in vitro. Two major deficiencies have existed in past organ culture models of tumor cell invasion. The first is a lack of quantitation. The second is the use of non-human organs which contain multiple types of host tissue. A majority of the previous invasion assays have been qualitative studies in which fragments of organs are admixed with tumor cells (Table 1) [1–13]. The extent of invasion was judged by making histologic sections of the tissues after various incubation times. These previous methods provide an ideal system for visualizing the microscopic interactions between malignant and benign tissue. Unfortunately, they cannot be used to routinely quantitate the rate of tumor cell invasion during different experimental treatments. Investigators such as Hart  and Poste  have therefore developed quantitative assays for invasion using labeled tumor cells cultured on chicken chorioallantoic membrane or within the lumen of a perfused canine vein. The proportion of labeled tumor cells which traverse these tissue barriers is quantified by counting the radioactivity on each side of the barrier. In the systems described by Poste et al. , tumor cells which traverse the tissues can be collected for further study. However, a disadvantage of these systems is the use of complex nonhuman organs. The chicken chorioallantoic membrane contains a series of multicellular and connective tissue layers (including endoderm and ectoderm) and is vascularized [3–8].
KeywordsMigration Proline Sarcoma Enzymatic Degradation Glutamine
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- 3.Easty DM, Easty GC: An in-vitro model for studying invasiveness. In: Organ culture in biomedical research, Balls M, Monnickendam M (eds). Cambridge: Cambridge University Press, 1976, pp 379–392.Google Scholar
- 5.Pourreau-Schneider N, Felix H, Haemmerli G: The role of cellular locomotion in leukemic infiltration. An organ culture study on penetration of L 5222 rat leukemia cells into the chick embryo mesonephros. Virch Arch B Cell Pathol 23:257–264, 1977.Google Scholar
- 7.Ticke A, Crawley A, Goodman M: Cell movement and the mechanism of invasiveness: a survey of the behavior of some normal and malignant cells implanted into the developing chick wing bud. J Cell Sci 31:293–322, 1978.Google Scholar
- 10.Mareel M, Kint J, Meyvisch C: Methods of study of the invasion of malignant C3H-mouse fibroblasts into embryonic chick heart In vitro. Virch Arch B Cell Pathol 30:95–111, 1979.Google Scholar
- 11.Maignan MF: Étude ultrastructurale des interactions entre des cellules normales ou malignes et le sac vitellin de rat, explanté In vitro. Biologie Cellulaire 35:229–232, 1979.Google Scholar
- 16.Vrako R: Basal lamina scaffold-anatomy and significance for maintenance of orderly tissue structure. Am J Pathol 77:314–346, 1974.Google Scholar
- 20.Wynn RM: Development and morphology of the amnion. In: Amniotic Fluid, Vol I, Natelson S, Scommegna A, Epstein MB (eds). New York: J. Wiley & Sons, 1973, pp 5–21.Google Scholar
- 24.Marchesi VT: Ultrastructural aspects of acute inflammation. Pathol Ann 5:343–353, 1970.Google Scholar
- 25.Grant L: The sticking and emigration of white blood cells in inflammation. In: The inflammatory process, Vol II, Zweifach BW, Grant L, Mc Cluskey RT (eds). New York: Academic Press, 1973, pp 245–249.Google Scholar
- 26.Ryan GB, Majno G: Acute inflammation: a review. Am J Pathol 86:185–276, 1977.Google Scholar
- 28.Niedel JE, Cuatrecasas P: Formyl peptide chemotactic receptors of leukocytes and macrophages. Curr Top in Cell Regul 17:137–170, 1980.Google Scholar
- 29.Becker EL: Some interrelations on neutrophil Chemotaxis, lysosomal enzyme secretion, and phagocytosis as revealed by synthetic peptides. Am J Pathol 85:383–394, 1976.Google Scholar