Abstract
In clinical routine work, a single hemocytometer count is widely used to determine whether a patient’s sperm concentration is normal or pathological. This procedure of diagnosis presents a complete overestimation of the method. There are two major variations when measuring sperm concentrations: 1) Biological variations which mainly depend on time of sexual abstinence (Schirren, 1972) and general health condition; 2) Variations due to the employed technique and the executing technician. Regarding biological variations, a single sperm count results in a confidence interval between 0.5 n and 2.3 n, i.e. For a measured concentration of 10 million spermatozoa/ml, the confidence interval varies between 5 million/ml (oligozoospermia) and 23 million/ml (normozoospermia)(Schwartz, 1974)Considering the analysis itself, a variation of 20% is due to the technique and 25% is due to the technician (Freund and Carol,1964)The two major sources of technical errors in the hemocytometer technique are inadequate mixing of the fresh sample and the necessary dilution with a spermicidal solution
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Referencess
Atherton RW (1977) Evaluation of sperm motility. In Hafez ESE ed. Techniques of human andrology, p 173–187. Elsevier/ North-Holland Biomedical Press.
Dahlberg B (1978) Infection of the male reproductive tract. Prog. Reprod Biol vol 3, pp 46–59. Basel: Karger.
Eliasson R (1971) Standards for investigation of human semen. Andrologie 3: 49.
Eliasson R, Maleika F Discussion at Xth World Congress on Fertility and Sterility, Madrid, 5–11 July 1980.
Freund M, Carol B (1964) Factors affecting hemocytometer counts of sperm concentration in human semen. J Reprod Fertil 8: 149.
Jondet M (1976) Congelation du sperme. Bull Acad Vet France 49: 373.
Makler A (1978a) A new chamber for rapid sperm count and motility estimation. Fertil Steril 30: 313.
Makler A (1978b) A new multiple exposure photography method for objective human spermatozoal motility determination. Fertil Steril 30: 192.
Makler A, Zaidise I, Paldi E, Brandes JM (1979a) Factors affecting sperm motility. I. In vitro change in motility with time after ejaculation. Fertil Steril 31: 147.
Makler A, Itskovitz J, Brandes JM, Paldi E (1979b) Sperm velocity and percentage of motility in 100 normospermic specimens analyzed by the multiple exposure photography MEP method. Fertil Steril 31: 155.
Makler A (1980) The improved ten-micrometer chamber for rapid sperm count and motility evaluation. Fertil Steril 33: 337.
Maleika F, Schäffer E, Lauritzen Ch (1980) A simple technique for objective measurement and evaluation of sperm motility and concentration (abstract) Arch Androl 5: 6. Paper presented at World Conference for instrumental insemination, in vitro fertilization and embryo transfer. Kiel, West Germany, 24–27 September 1980.
Schirren C (1972) Practical andrology. Berlin: Hartmann.
Schwartz D (1974) Spermiogramme et fertilité du couple. Contraception Fertilité Sexualité 6: 767.
Zaneveld LJD, Polakoski KL (1977) Collection and physical examination of the ejaculate. In Hafez ESE ed. Techniques of human andrology, pp 147–172. Elsevier/North-Holland Biomedical Press.
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© 1982 Martinus Nijhoff Publishers, The Hague
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Maleika, F., Angerer, B., Lauritzen, C. (1982). A Simple Technique for Rapid Sperm Count and Data Documentation. In: Hafez, E.S.E., Semm, K. (eds) Instrumental Insemination. Clinics in Andrology, vol 8. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-7467-8_15
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DOI: https://doi.org/10.1007/978-94-009-7467-8_15
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