Abstract
The use of tetrazolium salts to measure dehydrogenase activity (DA) of epilithic communities was more appropriate and sensitive than the oxygen change methods. While modifying the DA assay for estimation aerobic decomposition by periphyton, the effects of a defined medium on nonadapted periphyton was found to be insignificant. For DA analyses of periphyton we suggest using: triphenyl tetrazolium, an incubation temperature 28°C, a modified termination of enzyme reactions, and methods for avoiding the interference of photosynthetic pigments. The technique suggested gave reproductable results obtained from various samplings at the same place of a moderately polluted river during a fortnight within the range of variability of subsamples (10%). The DA of periphyton (per unit stone surface) from an area with a current velocity of 1.5 m s-1 was tenfold lower than that of the same water with a current velocity of 0.15 m s-1. The activity of the autumnal periphyton was twice greater than that during summer in areas shadded by forested banks. The DA was greater (up to 68 × 10-3 µmoles H2 h-1 cm-2) near organic pollution sources than a few kilometers downstream (min. 4.7 × 10-3 µmoles H2 h-1 cm-2).
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References
Altman, F.P., 1972. Quantitative dehydrogenase histochemistry with special reference to the pentose shunt dehydrogenases. Progr. Histochem. Cytochem. 4: 223–273.
Boyanovsky, B.B., 1979. Characteristics of Attached and Suspended Biomass in the Lower Part of the River Sazava and Measurement of Dehydrogenase Activity of River Periphyton. Ph. D. Thesis, Hydrobiol. Lab. Czechoslov. Acad, of Sci., Prague, (in Czech), 116 p.
Dockal, P., 1976. Slimes in Cooling Systems and Their Suppression. Water Research Institute, Prague, Czechoslovakia, 213 p.
Effenberger, M. & Zahradka, V., 1966. Vyzkum method stanoveni oxidacni richlosti smesi a enzymaticke aktivity kalu. Zaverecna sprava VUV, Praha, 69 p.
Ford, D.L., Yang, J.T., Eckenfelder, W.W., 1966. Dehydrogenase enzyme as a parameter of activated sludge activities. Proc. 21st Ing. Waste Conf. Purdue Univ., pp. 534–543.
Jambor, B., 1960. Tetrazolium Salze in der Bioligie. Gustav Fischer Verl. Jena, 151 p.
Hubena, J., 1975. Activity of the Activated Sludge…, Ph.D. Thesis, VSCHT, Prague, (in Czech), 139 pp.
Klapwijk, A., Drent, J., Steenworden, J.H.A.M., 1974. A modificated procedure for the TTC dehydrogenase test in activated sludge. Wat. Res., 8: 121–125.
Kun, E. & Abood, L.G., 1949. Colorimetric estimation of succinic dehydrogenase by triphenyltetrazolium chloride. Science 109: 144.
Lenhard, G., 1968. A standardized procedure for the determination of dehydrogenase activity in samples from anaerobic treatment systems. Wat. Res., 2: 161–167.
Puncochar, P., 1977. Bestimung von Bakterien im Aufwuchs (Periphyton) Proc. Ilnd. Int. Hydromicrobiol. Symp. Smolenice, 1975, ed. I. Daubner, Veda Vrl., Bratislava, pp. 73–78.
Simon, P. & Dore, M., 1977. Etude des paramètres du dosage des déhydrogenasés par le TTC applique aus boues activées. CEBEDEAU-BECEWA, 403: 252–265.
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© 1983 Dr W. Junk Publishers, The Hague
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Boyanovsky, B. (1983). A method for measuring the dehydrogenase activity of river periphyton. In: Wetzel, R.G. (eds) Periphyton of Freshwater Ecosystems. Developments in Hydrobiology, vol 17. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-7293-3_35
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DOI: https://doi.org/10.1007/978-94-009-7293-3_35
Publisher Name: Springer, Dordrecht
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