The application of a nuclear track autoradiographic technique to the study of periphyton photosynthesis

  • G. G. C. Robinson
  • E. Pip
Conference paper
Part of the Developments in Hydrobiology book series (DIHY, volume 17)

Abstract

The application of nuclear track autoradiography to the determination of inorganic carbon assimilation rates of fifteen periphytic algal species is described. Vertical profiles of carbon assimilation for each species following three periods of substrate colonization are presented, along with the contribution made by each species to total algal productivity. It is demonstrated that cellular carbon assimilation rates are a function of cell surface area and that, as a consequence, small periphytic algae have shorter generation times than large algae

Keywords

Biomass Cellulose Glycerine Iodine Phytoplankton 

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References

  1. American Public Health Association, 1971. Standard methods for the examination of water and wastewater. 13th ed. Washington, D.C.Google Scholar
  2. Harris, G.P. & Lott, J.N.A., 1973. Light intensity and photosynthetic rates in phytoplankton. J. Fish. Res. Bd. Can. 30: 1771–1778.CrossRefGoogle Scholar
  3. Hooper, N.M. & Robinson, G.G.C., 1976. Primary production of epiphytic algae in a marsh pond. Can. J. Bot. 54: 2810–2815.CrossRefGoogle Scholar
  4. Hooper-Reid, N.M. & Robinson, G.G.C., 1978. Seasonal dynamics of epiphytic algal growth in a marsh pond: productivity, standing crop, and community composition. Can. J. Bot. 56: 2434–2440.CrossRefGoogle Scholar
  5. Knoechel, R. & Kalff, J., 1976a. The applicability of grain density autoradiography to the quantitative determination of algal species production: a critique. Limnol. Oceanogr. 21: 583–590.CrossRefGoogle Scholar
  6. Knoechel, R. & Kalff, J., 1976b. Track autoradiography: a method for the determination of phytoplankton species pro-ductivity. Limnol. Oceanogr. 21: 590–596.CrossRefGoogle Scholar
  7. Knoechel, R. & Kalff, J., 1979. The advantages and disadvantages of grain density and track autoradiography. Limnol. Oceanogr. 24: 1170–1171.CrossRefGoogle Scholar
  8. Maquire, B. & Neill, W.E., 1971. Species and individual productivity in phytoplankton communities. Ecology 52: 903–907.CrossRefGoogle Scholar
  9. Paerl, H. W., 1977. Ultraphytoplankton biomass and production in some New Zealand lakes. N.Z.J. Mar. Freshwat. Res. 11: 297–305.CrossRefGoogle Scholar
  10. Paerl, H. W., 1978. Effectiveness of various counting methods in detecting viable phytoplankton. N.Z.J. Mar. Freshwat. Res. 12: 67–72.CrossRefGoogle Scholar
  11. Paerl, H.W. & Stull, E.A., 1979. In defense of grain density autoradiography. Limnol. Oceanogr. 24: 1166–1169.CrossRefGoogle Scholar
  12. Pip, E. & Robinson, G.G.C., 1982a. A study of the seasonal dynamics of three phycoperiphytic communities, using nuclear track autoradiography. I. Inorganic carbon uptake. Arch. Hydrobiol. 94: 341–371.Google Scholar
  13. Pip, E. & Robinson, G.G.C., 1982b. A study of the seasonal dynamics of three phycoperiphytic communities, using nuclear track autoradiography. II. Organic carbon uptake. Arch. Hydrobiol. 96: 47–64.Google Scholar
  14. Silver, M.W. & Davoll, P.J., 1978. Loss of 14C activity after chemical fixation of phytoplankton: error source for autoradiography and other productivity measurements. Limnol. Oceanogr. 23: 362–368.CrossRefGoogle Scholar
  15. Stull, E.A., de Amezaga E. & Goldman, C.R., 1973. The contribution of individual species of algae to primary productivity of Castlelake, California. Verh. Int. Verein. Limnol. 18: 1776–1783.Google Scholar
  16. Watt, W.D., 1971. Measuring the primary production rates of individual phytoplankton species in natural mixed populations. Deep-Sea Res. 18: 329–339.Google Scholar

Copyright information

© Dr W. Junk Publishers, The Hague 1983

Authors and Affiliations

  • G. G. C. Robinson
    • 1
  • E. Pip
    • 1
  1. 1.Department of BotanyUniversity of ManitobaWinnipegCanada

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