Direct examination of growing filaments to determine phosphate growth kinetics in Cladophora glomerata (L.) Kütz and Stigeoclonium tenue (Agardh) Kütz

  • Arno S. Rosemarin
Conference paper
Part of the Developments in Hydrobiology book series (DIHY, volume 17)


Semi-continuous (petri-plate) cultures were used for assessing growth kinetics in attached filaments of (Lake-Ontario derived) Cladophora glomerata and Stigeoclonium tenue relative to limiting levels of inorganic phosphate. Under enriched conditions, the filaments were allowed to produce zoospores which then attached to the base of the plastic petri-plates to produce germlings. Bottom attachment was achieved by exploiting positive phototaxis in providing bottom illumination. Six phosphate treatments, ranging from 0 to 2 µM PO4, were run for 15 days with daily replacement of medium. Cell counts were carried out on the same filaments each time, every two days, using an inverted light microscope. Specific growth rate was calculated as the doubling rate (of the number of cells per filament) per day. The Monod kinetics model was used to determine the growth kinetic coefficients. The results reinforce previous findings, derived using the Droop and Kilham variable internal stores model, that Stigeoclonium tenue outgrows Cladophora glomerata under conditions of limiting phosphate. This finding provides further evidence that the dominance of Cladophora glomerata in the North American Great Lakes may not be related strictly to the response to phosphate.


Specific Growth Rate Great Lake Filamentous Alga Inverted Light Microscope Phosphate Treatment 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Auer, M.T. & Canale, R.P., 1982a. Ecological studies and mathematical modeling of Cladophora in Lake Huron: 2. Phosphorus uptake kinetics. J. Great Lakes Res. 8 (1): 84–92.CrossRefGoogle Scholar
  2. Auer, M.T. & Canale, R.P., 1982b. Ecological studies and mathematical modeling of Cladophora in Lake Huron: 3. The dependence of growth rates on internal phosphorus pool size. J. Great Lakes Res. 8 (1): 93–99.CrossRefGoogle Scholar
  3. Chapman, A.R.O., 1973. Methods for macroscopic algae, pp. 87–104. In: Stein, J.R. (ed.) Handbook of Phycological Methods, Culture Methods and Growth Measurements. Cambridge Univ.Google Scholar
  4. Chapman, A.R.O., Markham, J.W. & Lüning, K., 1978. Effects of nitrate concentration on the growth and physiology of Laminaria saccharina (Phaeophyta) in culture. J. Phycol. 14 (2): 195–198.CrossRefGoogle Scholar
  5. Cox, E.R. & Bold, H.C., 1966. Taxonomic investigations of Stigeoclonium. Phycol. Stud., 7: 7–167.Google Scholar
  6. Droop, M.R., 1968. Vitamin B12 and marine ecology. IV. The kinetics of uptake, growth and inhibition in Monochrysis lutheri. J. Mar. Biol. Assoc. U.K. 48: 689–733.CrossRefGoogle Scholar
  7. Francke, J.A. & Cate, H.J. ten, 1980. Ecotypic differentation in response to nutritional factors in the algal genus Stigeoclonium Kütz. (Chlorophyceae). Br. Phycol. J. 15: 343–355.CrossRefGoogle Scholar
  8. Gerloff, G.C. & Fitzgerald, G.P., 1976. The nutrition of Great Lakes Cladophora. EPA Report 600/3-76-044.Google Scholar
  9. Graham, J.M., Auer, M.T., Canale, R.P. & Hoffmann, J.P., 1982. Ecological studies and mathematical modeling of Cladophora in Lake Huron: 4. Photosynthesis and respiration as functions of light and temperature. J. Great Lakes Res. 8 (1): 100–111.CrossRefGoogle Scholar
  10. Guillard, R.R.L., 1973. Methods for microflagellates and nannoplankton, pp. 69–85. In: Handbook of Phycological Methods, Culture Methods and Growth Measurements (J.R. Stein, ed.) Cambridge Univ.Google Scholar
  11. Haines, K.C. & Wheeler, P.A., 1978. Ammonium and nitrate uptake by the marine macrophytes Hypnea musciformis (Rhodophyta) and Macrocystis pyrifera (Phaeophyta). J. Phycol. 14: 319–324.CrossRefGoogle Scholar
  12. Hanisak, M.D. & Harlin, M.M., 1978. Uptake of inorganic nitrogen by Codium fragile subsp. tomentosoides (Chlorophyta). J. Phycol. 14: 450–454.CrossRefGoogle Scholar
  13. Healey, F.P., 1980. Slope of the Monod equation as an indicator of advantage in nutrient competition. Microb. Ecol. 5: 281–286.CrossRefGoogle Scholar
  14. Horner, R.R. & Welch, E.B., 1981. Stream periphyton development in relation to current velocity and nutrients. Can. J. Fish. Aquat. Sci. 38: 449–457.CrossRefGoogle Scholar
  15. Indegaard, M. & Jensen, A., 1981. Nitrate and phosphate uptake in small populations of Laminaria digitata (Phaeophyceae). pp. 411–417. In: Xth international Seaweed Symposium. Berlin: Walter de Gruyter & Co.Google Scholar
  16. Kilham, S.S., 1978. Nutrient kinetics of freshwater planktonic algae using batch and semicontinuous methods. Mitt. Internat. Verein. Limnol. 21: 147–157.Google Scholar
  17. Lapointe, B.E. & Tenore, K.R., 1981. Experimental outdoor studies with Ulva fasciata Delile. 1. Interaction of light and nitrogen on nutrient uptake, growth, and biochemical composition. J. exp. mar. Biol. Ecol. 53: 135–152.CrossRefGoogle Scholar
  18. Mantai, K.E., Garwood, P.E. & Peglowski, L.E., 1982. Environmental factors controlling physiological changes in Cladophora in Lake Erie. J. Great Lakes Res. 8 (1): 61–65.CrossRefGoogle Scholar
  19. McCarthy, J.J., 1981. The kinetics of nutrient utilization, pp. 211–233. In: Physiological bases of phytoplankton ecology. ( Platt, T. ed.). Can. Bull. Fish. Aquat. Sci. 210.Google Scholar
  20. Monod, J., 1942. Recherches sur la Croissance des Cultures bactériennes. Paris: Hermann.Google Scholar
  21. Murphy, J. & Riley, J., 1962. A modified single solution method for the determination of phosphate in natural waters. Anal. Chim. Acta 27: 31–36.CrossRefGoogle Scholar
  22. Reynolds, N., 1950. Methods of culturing epiphytic algae. New Phytologist 49: 155–162.CrossRefGoogle Scholar
  23. Rhee, G-Y., 1980. Continuous culture in phytoplankton ecology. pp. 151–203. In: Advances in Aquatic Microbiology Vol. 2 (Droop, M.R. and Jannasch, H.W. ed.). London: Academic Press.Google Scholar
  24. Rosemarin, A. S., 1982. Phosphorus nutrition of two potentially competing filamentous algae, Cladophora glomerata (L.) Kütz and Stigeoclonium tenue (Agardh) Kütz. from Lake Ontario. J. Great Lakes Res. 8 (1): 66–72.CrossRefGoogle Scholar
  25. Spencer, D.F. & Lembi, C.A., 1981. Factors regulating the spatial distribution of the filamentous alga Pithophora oedogonium (Chlorophyceae) in an Indiana Lake. J. Phycol. 17: 168–173.CrossRefGoogle Scholar
  26. Trotter, D.M. & Hendricks, A.C., 1979. Attached, filamentous algal communities, pp. 58–69. In: Methods and measurements of periphyton communities: a review ( Weitzel, R.L. ed.) Philadelphia: American Society for Testing and Materials.CrossRefGoogle Scholar
  27. Venkataraman, G.S., 1969. The Cultivation of Algae. New Delhi: Indian Council of Agriculture Research.Google Scholar

Copyright information

© Dr W. Junk Publishers, The Hague 1983

Authors and Affiliations

  • Arno S. Rosemarin
    • 1
  1. 1.Department of BiologyQueens UniversityKingstonCanada

Personalised recommendations