Environmental factors affecting hatching of rotifer (Brachionus plicatilis) resting eggs
Hatching experiments were carried out on a population of Brachionus plicatilis (Dor strain) resting eggs produced in batch laboratory cultures under controlled conditions and then stored for at least one month at 4°C in the dark. Light was found to be obligatory for termination of dormancy. Over the temperature range of 10–30°C (at 9.0‰ salinity), hatching was optimal (40–70%) at 10–15°C and decreased linearly with the rise in incubation temperature. Resting eggs incubated over a salinity range of 9–40‰ (at 15°C) showed optimal hatching at 16‰. Incubation of resting eggs in distilled water permitted normal embryonic development, but neonates died at eclosion. Presence of algae, Chlorella stigmatophora (0.5 × 106 cell ml−1), was found to aid hatching.
Keywordsrotifers resting eggs hatching temperature salinity light algae
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