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Two Dimensional NMR Investigation of the Structural Properties of DNAS

  • Conference paper
Nucleic Acids: The Vectors of Life

Part of the book series: The Jerusalem Symposia on Quantum Chemistry and Biochemistry ((JSQC,volume 16))

Abstract

We describe the use of 2D NOE and truncated driven NOE (TNOE) spectroscopy to investigate structural features of three alternating homopolymer systems; poly(dA-dT), poly(dG-dC) (B- and Z-forms), and poly(dI-dC). Poly(dA-dT) and poly(dI-dC) have Watson-Crick base pairing, not Hoogsteen pairing as recently proposed for fibers. The B-form of poly(dG-dC), poly(dA-dT) and poly(dI-dC) exhibit qualitative structural features expected for B-family DNA. Specifically, all have anti-glycosidic torsional angles. This is contrasted with results from Z-form poly(dG-dC) which exhibits a syn-torsional angle as well as other differences in the 2D NOE spectrum. 2D NOE measurements on the decamer d(ATATCGATAT)2 in H2O were used to delineate the various spin lattice relaxation pathways (dipolar, exchange) of the imino protons in this molecule.

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Author information

Authors and Affiliations

  1. Department of Chemistry, B-014, University of California-San Diego, La Jolla, CA, 92093, USA

    David R. Kearns, Peter A. Mirau, Nuria Assa-Munt & Ronald W. Behling

Authors
  1. David R. Kearns
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  2. Peter A. Mirau
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  3. Nuria Assa-Munt
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  4. Ronald W. Behling
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Editor information

Editors and Affiliations

  1. Institut de Biologie Physico-Chimique (Fondation Edmond de Rothschild), Universite Pierre et Marie Curie (Paris VI), Paris, France

    Bernard Pullman

  2. Department of Chemistry, Tel-Aviv University, Tel-Aviv, Israel

    Joshua Jortner

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© 1983 D. Reidel Publishing Company

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Kearns, D.R., Mirau, P.A., Assa-Munt, N., Behling, R.W. (1983). Two Dimensional NMR Investigation of the Structural Properties of DNAS. In: Pullman, B., Jortner, J. (eds) Nucleic Acids: The Vectors of Life. The Jerusalem Symposia on Quantum Chemistry and Biochemistry, vol 16. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-7225-4_9

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  • DOI: https://doi.org/10.1007/978-94-009-7225-4_9

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-009-7227-8

  • Online ISBN: 978-94-009-7225-4

  • eBook Packages: Springer Book Archive

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