Skip to main content
SpringerLink
Log in

Dynamic Structure of Z-DNA

  • Conference paper
Nucleic Acids: The Vectors of Life

Part of the book series: The Jerusalem Symposia on Quantum Chemistry and Biochemistry ((JSQC,volume 16))

Abstract

We first show that under topological constraints segments of natural DNA containing (A.T) and (G.C) base pairs can adopt the Z conformation. This is deduced from the titration of form V DNA by the antibodies to Z-DNA. We then report some results on exchange kinetics of the protons involved in hydrogen bonds between base pairs of poly(dG-m5dC).poly(dG-m5dC), in Z conformation. There are two classes of protons, a fast one the exchange half-time of which is 40 mn and a slow one the exchange half-time of which is 960 mn. These values are deduced from infrared absorption measurements in 2H2O and H2O. These half-times are in good agreement with those determined for several alternating polynucleotides in Z-form and confirm the difference between the dynamic structure of B and Z-forms. Finally, we have studied the thermal stability of (m5dC-dG)3 by 31P nuclear magnetic resonance, circular dichroism and ultra-violet absorption. In 3 M NaC1O4, (m5dC-dG)3 has the Z conformation. The melting curve of the hexanucleotide is biphasic. The first transition occurs over a large range of temperature. It corresponds to a conformational change due to an intramolecular non-cooperative process. This new conformation is not the B-form. The second transition is cooperative and corresponds to the melting of a double helix into single strands.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 39.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 54.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Wang, A.H.J., Quigley, G.J., Kolpak, F.J., Crawford, J.L., van Boom, J.H., van der Marel, G. and Rich, A.: 1979, Nature 282, pp 680–686.

    Article  CAS  Google Scholar 

  2. Drew, H., Takano, T., Tanaka, S., Itakura, K. and Dickerson, R.E.: 1980, Nature 286, pp 567–573.

    Article  CAS  Google Scholar 

  3. Rich, A.: 1983, in Structure, Dynamics, Interactions and Evolution of Biological Macromolecules, Hélène, C., ed., D. Reidel Publishing Company, Dordrecht, pp 3–21.

    Chapter  Google Scholar 

  4. Leng, M.: 1983, in Structure, Dynamics, Interactions and Evolution of Biological Macromolecules, Hélène, C., ed., D. Reidel Publishing Company, Dordrecht, pp 45–53.

    Chapter  Google Scholar 

  5. Lafer, E.M., Möller, A., Nordheim, A., Stollar, D.B. and Rich, A.: 1981, Proc. Natl. Acad. Sci. USA 78, pp. 3546–3550.

    Article  CAS  Google Scholar 

  6. Malfoy, B. and Leng, M.: 1981, FEBS Letters 132, pp 45–48.

    Article  CAS  Google Scholar 

  7. Nordheim, A., Pardue, M.L., Lafer, E.M., Möller, A., Stollar, D.B. and Rich, A: 1981, Nature 294, pp 417–422.

    Article  CAS  Google Scholar 

  8. Leng, M., Hartmann, B., Malfoy, B., Pilet, J., Ramstein, J. and Sage, E.: 1983, in 47th Symposium on Quantitative Biology, Cold Spring Harbor, in press.

    Google Scholar 

  9. Lemeunier, F., Derbin, C., Malfoy, B., Leng, M. and Taillandier, E.: 1982, Exp. Cell. Res. 141, pp 508–513.

    Article  CAS  Google Scholar 

  10. Lipps, H.J., Nordheim, A., Lafer, E.M., Ammermann, D., Stollar, D.B. and Rich, A.: 1983, Cell 32, pp 435–441.

    Article  CAS  Google Scholar 

  11. Morgenegg, G., Celio, M.R., Malfoy, B., Leng, M. and Kuenzle, C.C.: 1983, Nature, in press.

    Google Scholar 

  12. Stettler, U.H., Weber, H., Koller, Th. and Weissmann, C.: 1979, J. Mol. Biol. 131, pp 21–40.

    Article  CAS  Google Scholar 

  13. Lang, M.C., Malfoy, B., Freund, A.M., Daune, M. and Leng, M.: 1982, The Embo J. 1, pp 1149–1153.

    CAS  Google Scholar 

  14. Pullman, B., Pullman, A. and Lavery, R.: 1983, in Structure, Dynamics, Interactions and Evolution of Biological Macromolecules, Hélène, C., ed., Dordrecht, D. Reidel Publishing Company, pp 23–44.

    Book  Google Scholar 

  15. Leng, M., Freund, A.M., Malfoy, B., Malinge, J.M., Pilet, J. and Rio, P.: 1983, in Cellular Responses to DNA Damage. UCLA Symposia on Molecular and Cellular Biology, New Series, Friedberg, E.C. and Bridges, B.R., eds, Alan R. Liss, Inc. New York, vol. 11, in press.

    Google Scholar 

  16. Fujii, S., Wang, A.H.J., van der Marel, G., van Boom, J.H. and Rich, A.: 1982, Nucleic Acids Res. 10, pp 7879–7892.

    Article  CAS  Google Scholar 

  17. Thamann, T.J., Lord, R.C., Wang, A.H.J. and Rich, A.: 1981, Nucleic Acids Res. 9, pp 5443–5457.

    Article  CAS  Google Scholar 

  18. Quadrifoglio, F., Manzini, G., Vasser, M., Dinkelspiel, K. and Crea, R.: 1981, Nucleic Acids Res. 9, pp 2195–2206.

    Article  CAS  Google Scholar 

  19. Malfoy, B., Hartmann, B. and Leng, M.: 1981, Nucleic Acids Res. 9, pp 5659–5669.

    Article  CAS  Google Scholar 

  20. Malfoy, B., Rousseau, N. and Leng, M.: 1982, Biochemistry 21, pp 5463–5467.

    Article  CAS  Google Scholar 

  21. Nordheim, A., Lafer, E.M., Peck, L.J., Wang, J.C., Stollar, D.B. and Rich, A.: 1982, Cell 31, pp 309–318.

    Article  CAS  Google Scholar 

  22. Peck, L.J., Nordheim, A., Rich, A. and Wang, J.C.: 1982, Proc. Natl. Acad. Sci. USA 79, pp 4560–4564.

    Article  CAS  Google Scholar 

  23. Singleton, C.K., Klysik, J., Stirdivant, S.M. and Wells, R.P.: 1982, Nature 299, pp 312–316.

    Article  CAS  Google Scholar 

  24. Behe, M. and Felsenfeld, G.: 1981, Proc. Natl. Acad. Sci. USA 78, pp 1619–1623.

    Article  CAS  Google Scholar 

  25. Pohl, F.M. and Jovin, T.M.: 1972, J. Mol. Biol. 67, pp 375–396.

    Article  CAS  Google Scholar 

  26. Teitelbaum, H. and Englander, S.W.: 1975, J. Mol. Biol. 92, pp 55–78.

    Article  CAS  Google Scholar 

  27. Teitelbaum, H. and Englander, S.W.: 1975, J. Mol. Biol. 92, pp 79–92.

    Article  CAS  Google Scholar 

  28. Ramstein, J. and Leng, M.: 1980, Nature 288, pp 413–414.

    Article  CAS  Google Scholar 

  29. Hartmann, B., Pilet, J., Ptak, M., Ramstein, J., Malfoy, B. and Leng, M.: 1982, Nucleic Acids Res. 10, pp 3261–3277.

    Article  CAS  Google Scholar 

  30. Pilet, J. and Leng, M.: 1982, Proc. Natl. Acad. Sci. USA 79, pp 26–30.

    Article  CAS  Google Scholar 

  31. Rio, P., Loukakou, P. and Leng, M., 1983, Nucleic Acids Res., submitted.

    Google Scholar 

  32. Miller, J.A. and Miller, E.C.: 1979, in Environmental Carcinogenesis, Occurrence, Risk, Evaluation and Mechanism, Emmelot, P. and Kriek, E., eds, Elsevier/North Holland Biomedical Press, p 25–50.

    Google Scholar 

  33. Lavery, R. and Pullman, B.: 1981, Nucleic Acids Res. 9, pp 4677–4688.

    Article  CAS  Google Scholar 

  34. Spodheim-Maurizot, M., Saint-Ruf, G. and Leng, M.: 1979, Nucleic Acids Res.6, PP 1683–1694.

    Google Scholar 

  35. Pohl, F.M.: 1974, Eur. J. Biochem. 42, pp 495–504.

    Article  CAS  Google Scholar 

  36. Patel, D.J., Canuel, L.L. and Pohl, F.M.: 1979, Proc. Natl. Acad. Sci. USA 76, pp 2508–2511.

    Article  CAS  Google Scholar 

  37. Hogan, M.E. and Jardetzky, O.: 1980, Biochemistry 19, pp 3460–3465.

    Article  CAS  Google Scholar 

  38. Brahms, S., Vergne, J., Brahms, J.G., Dicapua, E., Bucher, P.H. and Koller, Th.: 1982, J. Mol. Biol. 162, pp 473–493.

    Article  CAS  Google Scholar 

  39. Pohl, F.M., Thomae, R. and Dicapua, E.: 1982, Nature 300, pp 545–546

    Article  CAS  Google Scholar 

  40. Jovin, T.M., vand de Sande, J.H., Zarling, D.A., Arndt-Jovin, D.J., Eckstein, F., Fuldner, H.H., Greider, C., Grieger, I., Hamori, E., Kalisch, B., McIntosh, L.P. and Robert-Nicoud, M.: 1983, 47th Symposium on Quantitative Biology, Cold Spring Harbor, in press.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Centre de Biophysique Moléculaire, C.N.R.S., 1A, avenue de la Recherche Scientifique, 45045, Orléans cedex, France

    M. Leng, B. Hartmann, B. Malfoy, J. Pilet & M. Ptak

Authors
  1. M. Leng
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. B. Hartmann
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. B. Malfoy
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. J. Pilet
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. M. Ptak
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Institut de Biologie Physico-Chimique (Fondation Edmond de Rothschild), Universite Pierre et Marie Curie (Paris VI), Paris, France

    Bernard Pullman

  2. Department of Chemistry, Tel-Aviv University, Tel-Aviv, Israel

    Joshua Jortner

Rights and permissions

Reprints and permissions

Copyright information

© 1983 D. Reidel Publishing Company

About this paper

Cite this paper

Leng, M., Hartmann, B., Malfoy, B., Pilet, J., Ptak, M. (1983). Dynamic Structure of Z-DNA. In: Pullman, B., Jortner, J. (eds) Nucleic Acids: The Vectors of Life. The Jerusalem Symposia on Quantum Chemistry and Biochemistry, vol 16. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-7225-4_4

Download citation

  • DOI: https://doi.org/10.1007/978-94-009-7225-4_4

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-009-7227-8

  • Online ISBN: 978-94-009-7225-4

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation