Skip to main content
SpringerLink
Log in

Nucleosomal DNA Structure

  • Conference paper
Nucleic Acids: The Vectors of Life

Part of the book series: The Jerusalem Symposia on Quantum Chemistry and Biochemistry ((JSQC,volume 16))

Abstract

The helical repeat of the DNA in the nucleosome is evaluated by three independent methods: from periodical distribution of the dinucleotides along known nucleosomal DNA sequences, from topology of DNA super-helix in the nucleosome and from analysis of accessibility of DNA in the nucleosome to DNase I, all giving similar results. All previous estimates are critically reviewed and the average figure is obtained, 10.38 ± 0.02 base-pairs per turn, based on five independent estimates. Experimental data available indicating that the free nucleosomal DNA is inherently curved, are reviewed. A novel approach to the higher order structure of chromatin is discussed which is based on steric exclusion effects imposing specific restrictions on the relative spatial positioning of the neighboring nucleosomes.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 39.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 54.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Lohr, D., and van Holde, K.E.: 1979, Proc.Natl.Acad.Sci.USA 76, pp. 6326–6330.

    Article  CAS  Google Scholar 

  2. Karpov, V.L., Bavykin, S.G., Preobrazhenskaya, O.V., Belyaysky, A.V., and Mirzabekov, A.D.: 1982, Nucl.Acids Res. 10, pp. 4321–4337.

    Article  CAS  Google Scholar 

  3. Strauss, F., and Prunell, A.: 1983, EMBO J. 2, pp. 51–56.

    CAS  Google Scholar 

  4. Mengeritsky, G., and Trifonov, E.N.: submitted.

    Google Scholar 

  5. Ulanovsky, L., and Trifonov, E.N.: submitted.

    Google Scholar 

  6. Levitt, M.: 1978, Proc.Natl.Acad.Sci.USA 75, pp. 640–644.

    Article  CAS  Google Scholar 

  7. Klug, A., and Lutter, L.C.: 1981, Nucl.Acids Res. 9, pp. 4267–4283.

    Article  CAS  Google Scholar 

  8. Prunell, A., Kornberg, R., Lutter, L., Klug, A., Levitt, M., and Crick, F.: 1979, Science 204, pp. 855–858.

    Article  CAS  Google Scholar 

  9. Trifonov, E. and Bettecken, T.: 1979, Biochemistry 18, pp. 454–456.

    Article  CAS  Google Scholar 

  10. Trifonov, E.N., and Sussman, J.L.: 1980, Proc.Natl.Acad.Sci.USA 77, pp. 3816–3820.

    Article  CAS  Google Scholar 

  11. Trifonov, E.N.: 1981, in “Structural Aspects of Recognition and Assembly in Biol.Macromolecules” (Ed. M. Balaban, J. Sussman, W. Traub and A. Yonath, Balaban ISI, Rehovot-Philadelphia, pp. 711–720.

    Google Scholar 

  12. Trifonov, E.N.: 1981, in “International Cell Biology 1980–1981” (Ed. H. Schweiger, Springer-Verlag ), pp. 128–138.

    Google Scholar 

  13. Trifonov, E.N.: 1980, Nucl.Acids Res. 8, pp. 4041–4053.

    Article  CAS  Google Scholar 

  14. Chao, M.V., Gralla, J., and Martinson, G.: 1970, Biochemistry 18, pp. 1068–1074.

    Article  Google Scholar 

  15. Igo-Kemenes, T., Omori, A., and Zachau, H.G.: 1980, Nucl.Acids Res. 8, pp. 5377–5390.

    Article  CAS  Google Scholar 

  16. Igo-Kemenes, T., and Seligman, H.: personal communication.

    Google Scholar 

  17. Wu, K.C., and Varshaysky, A.: submitted.

    Google Scholar 

  18. Gottesfeld, J.: submitted.

    Google Scholar 

  19. Wartenfeld, R., Mengeritsky, G., and Trifonov, E.N.: in preparation.

    Google Scholar 

  20. Zhurkin, V.B.: 1981, Nucl.Acids Res. 9, pp. 1963–1971.

    Article  CAS  Google Scholar 

  21. Sussman, J.L., and Trifonov, E.N.: 1978, Proc.Natl.Acad.Sci.USA 75, pp. 103–107.

    Article  CAS  Google Scholar 

  22. Trifonov, E.N.: 1978, Nucl. Acids Res. 5, pp. 1371–1380.

    Article  CAS  Google Scholar 

  23. Bentley, G.A., Finch, J.T., and Lewit-Bentley, A.: 1981, J.Mol.Biol. 145, pp. 771–784.

    Article  CAS  Google Scholar 

  24. Crick, F.H.C.: 1976, Proc.Natl.Acad.Sci.USA 73, pp. 2639–2643.

    Article  CAS  Google Scholar 

  25. Peck, L.J., and Wang, J.C.: 1981, Nature 292, pp. 375–378.

    Article  CAS  Google Scholar 

  26. Strauss, F., Gaillard, C., and Prunell, A.: 1981, Eur.J.Bioch. 118, pp. 215–222.

    Article  CAS  Google Scholar 

  27. Lutter, L.: 1979, Nucl.Acids Res. 6, pp. 41–56.

    Article  CAS  Google Scholar 

  28. Trifonov, E.N.: submitted.

    Google Scholar 

  29. Müller, U., Zentgraf, H., Eicken, I., and Keller, W.: 1978, Science 201, pp. 406–415.

    Article  Google Scholar 

  30. Shishido, K.: 1980, FEBS Lett. 111, pp. 333–336.

    Article  CAS  Google Scholar 

  31. Trifonov, E.N.: 1983: Cold Spring Harb.Symp.Quant.Biol. 47, in press

    Google Scholar 

  32. Mengeritsky, G., and Trifonov, E.N.: in preparation.

    Google Scholar 

  33. Rhodes, D., and Klug, A.: 1980, Nature 286, pp. 573–578.

    Article  CAS  Google Scholar 

  34. Kabsch, W., Sander, C., and Trifonov, E.N.: 1982, Nucl.Acids Res. 10, pp. 1097–1104.

    Article  CAS  Google Scholar 

  35. Lee, C.-H., and Charney, E.: 1982, J.Mol.Biol. 161, pp. 289–304.

    Article  CAS  Google Scholar 

  36. Marini, J.C., Levene, S.D., Crothers, D.M., and Englund, P.T.: 1982, Proc.Natl.Acad.Sci.USA 79, pp. 7664–7668.

    Article  CAS  Google Scholar 

  37. Ross, W., Shulman, M., and Landy, A.: 1982, J.Mol.Biol. 156, pp. 505–522.

    Article  CAS  Google Scholar 

  38. Ross, W., and Landy, A.: 1982, J.Mol.Biol. 156, pp. 523–529.

    Article  CAS  Google Scholar 

  39. Simpson, R.T., and Stafford, D.W.: 1983, Proc.Natl.Acad.Sci.USA 80, pp. 51–55.

    Article  CAS  Google Scholar 

  40. Ulyanov, N.B., Zhurkin, V.B., and Ivanov, V.I.: 1982, Stud.bioph. 87, pp. 99–100.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Polymer Research, The Weizmann Institute of Science, Rehovot, Israel, 76100

    E. N. Trifonov

Authors
  1. E. N. Trifonov
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Institut de Biologie Physico-Chimique (Fondation Edmond de Rothschild), Universite Pierre et Marie Curie (Paris VI), Paris, France

    Bernard Pullman

  2. Department of Chemistry, Tel-Aviv University, Tel-Aviv, Israel

    Joshua Jortner

Rights and permissions

Reprints and permissions

Copyright information

© 1983 D. Reidel Publishing Company

About this paper

Cite this paper

Trifonov, E.N. (1983). Nucleosomal DNA Structure. In: Pullman, B., Jortner, J. (eds) Nucleic Acids: The Vectors of Life. The Jerusalem Symposia on Quantum Chemistry and Biochemistry, vol 16. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-7225-4_29

Download citation

  • DOI: https://doi.org/10.1007/978-94-009-7225-4_29

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-009-7227-8

  • Online ISBN: 978-94-009-7225-4

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation