Skip to main content

Analytical Aspects of Monitoring Diethylstilbestrol and Related Anabolic Compounds in Stored Samples of Different Origin

  • Chapter
Environmental Specimen Banking and Monitoring as Related to Banking

Abstract

It is well known for many years that naturally occurring sex hormones and numerous synthetic analogues are stimulating the biosynthesis of proteins thus being most frequently used as growth-promoting agents in beef, cattle and poultry meat production (1). The range of anabolic compounds applied covers androgens, estrogens and gestagenes, the synthetic stilbene derivatives such as diethylstilbestrol (DES), dienestrol (DIES) and hexestrol (HEX) being the most commonly administered drugs (2). Typical representatives, their origin and the correlation between chemical structure and biochemical activity are presented in fig. 1. Within that frame the arrangement of the synthetic compounds is based on their structural analogy to the corresponding natural steroid hormones with equivalent biochemical properties.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 169.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 219.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 219.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Kochakian CD, Murlin JR. 1935. The Effect of Male Hormone on the Protein and Energy Metabolism of Castrate Dogs. J. Nutr. 10, 437.

    CAS  Google Scholar 

  2. Hoffmann B. 1979. Rückstandsprobleme bei der Hormonanwen- dung. Der praktische Tierarzt 60, 609–612.

    Google Scholar 

  3. Vanderwal P. 1976. General Aspects of the Effectiveness of Anabolic Agents in Increasing Protein Production in Farm Animals, in Particular in Bull Calves. In “Anabolic Agents in Animal Production”. Korte F, Coulston F. Editors. Georg Thieme Verlag, Stuttgart, p. 60–78.

    Google Scholar 

  4. Karg H. 1980. Erlaubte und unerlaubte Anabolika in der Kälbermast; Risiken und Kontrollmöglichkeiten. Kraft- futter, 63, 426–429.

    Google Scholar 

  5. McMartin KE, Kennedy KA, Grennspan P, Alam SN, Greiner P, Yam J. 1978. Diethylstilbestrol; A Review of its Toxicity and Use as a Growth Promotant in Food Producing Animals. J. Environm. Path. Toxicol., 1, 279–313.

    CAS  Google Scholar 

  6. Marx JL, Weidenfeld J, Carter P, Reinhold VR. 1978. Estrogens; Hormones’ Link to Cancer. Science, 202, 1270–1271

    Article  PubMed  CAS  Google Scholar 

  7. Dürbeck HW. 1979. Naturally Occuring Steroids and Synthetic Hormones as Sensitive Monitoring Compounds for the Suitability of Pretreatment Procedures in Specimen Banking and for the Long-Term Stability of Stored Biological Samples. In: Monitoring Environmental Materials and Specimen Banking. Luepke NP. Editor. Martinus Nijhoff Publishers, The Hague, p. 184–197.

    Google Scholar 

  8. Dürbeck HW, Frischkorn CGB, Frischkorn HE. 1978. Untersu- chungen zum Stoffwechselverhalten des Dianabol. Ein Beitrag zur Bestimmung von Anabolika. Dt. Zeitschr. Sportmed., 29, 97–103.

    Google Scholar 

  9. Dürbeck HW, Büker I. 1980. Studies on Anabolic Steroids. The Mass Spectra of 17α-Methyl-17β-hydroxy-1,4-androstadien- 3-one (Dianabol) and its Metabolites. Biomed. Mass Spectrom., 7, 437–445.

    Article  PubMed  Google Scholar 

  10. Frischkorn CGB, Dürbeck HW. 1979. Bestimmung von anabolen Steroiden und ihren Metaboliten in biologischen Materialien. Fresenius Z. Anal. Chem., 290, 160–161.

    Article  Google Scholar 

  11. Hoffmann B. 1978. Use of Radioimmunoassay for Monitoring Hormonal Residues in Edible Animal Products. J. Ass. Off. Anal. Chem., 61, 1263–1273.

    CAS  Google Scholar 

  12. Hoffmann B. Private communication.

    Google Scholar 

  13. Frischkorn CGB, Smyth MR, Frischkorn HE, Golimowski J. 1980. Spurenbestimmung östrogenwirkender Masthilfsmittel in Fleisch durch HPLC mit voltammetrischer Detektion. Fresenius Z. Anal. Chem., 300, 407–412.

    Article  CAS  Google Scholar 

  14. Smyth MR, Frischkorn CGB. 1980. Trace Determination of Some Phenolic Growth Promoting Hormones in Meat by High Performance Liquid Chromatography. Fresenius Z. Anal. Chem., 301, 220–223.

    Article  CAS  Google Scholar 

  15. Verbeke R. 1979. Sensitive Multi-Residue Method for Detection of Anabolics in Urine and in Tissues of Slaughtered Animals. J. Chromatogr., 177, 69–84.

    Article  PubMed  CAS  Google Scholar 

  16. Dürbeck HW, Büker I, Scheulen B, Telin B. 1978. GC and Capillary GC/MS-Determination of Synthetic Anabolic Steroids: Methandienone and its Metabolites. J. Chromatogr., 167, 117–124.

    Article  PubMed  Google Scholar 

  17. Dürbeck HW, Büker I, Leymann W. 1978. Ein modifiziertes Interface für die GC/MS-Kopplung von Glaskapillarsäulen. Chromatographia 11, 372–375

    Article  Google Scholar 

  18. Frischkorn CGB, Schlimper H. 1982. A Cheap and Simple Method for the Preparation of “HPLC-Pure” Water at the Laboratory Scale. Fresenius Z. Anal. Chem. (in press)

    Google Scholar 

  19. Frischkorn CGB, Frischkorn HE, Ohst I. 1978. Der simultane ppm-Nachweis des anabol wirkenden Zeranol und seines Metaboliten Zearalanon in Fleisch mittels hochauflösender Flüssigkeitschromatographie. Z. Lebensmittel-Unters. Forschung, 167, 7–10.

    Article  CAS  Google Scholar 

  20. Dürbeck HW, Frischkorn CGB. 1981. Anabolika in der Tier- mast - Nutzen und Risiko Jahresbericht 1980/81 der Kernforschungsanlage Jülich. p. 67–78

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 1984 Martinus Nijhoff Publishers, Boston

About this chapter

Cite this chapter

Dürbeck, H.W. (1984). Analytical Aspects of Monitoring Diethylstilbestrol and Related Anabolic Compounds in Stored Samples of Different Origin. In: Lewis, R.A., Stein, N., Lewis, C.W. (eds) Environmental Specimen Banking and Monitoring as Related to Banking. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-6765-6_25

Download citation

  • DOI: https://doi.org/10.1007/978-94-009-6765-6_25

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-009-6767-0

  • Online ISBN: 978-94-009-6765-6

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics