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Spectroscopy of Biological Molecules pp 347–372Cite as

Static and Time-Resolved Infrared Difference Spectroscopy Applied to Rhodopsin and Bacteriorhodopsin

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Part of the book series: NATO ASI Series ((ASIC,volume 139))

Abstract

The principles of static and time-resolved infrared difference spectroscopy and its application to biochemical systems is described. Important experimental aspects of both methods are discussed. The application of the methods to the investigation of the light-induced reactions of bacteriorhodopsin and rhodopsin is presented. The results demonstrate, that the method allows the detection of molecular changes of the chromophores as well as of the protein, and that it is especially sensitive for the retinylidene Schiff base binding site of these retinal-proteins. Regarding the chromophore similar results, in some cases also complementary, are obtained as in resonance Raman spectra. Completely new information is obtained with respect to molecular events in the protein.

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Author information

Authors and Affiliations

  1. Institut für Biophysik und Strahlenbiologie, Freiburg im Br., Germany

    F. Siebert

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  1. F. Siebert
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Editor information

Editors and Affiliations

  1. Department of Chemistry, University of Montreal, Montreal, Quebec, Canada

    Camille Sandorfy  & Theophile Theophanides  & 

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© 1984 D. Reidel Publishing Company

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Siebert, F. (1984). Static and Time-Resolved Infrared Difference Spectroscopy Applied to Rhodopsin and Bacteriorhodopsin. In: Sandorfy, C., Theophanides, T. (eds) Spectroscopy of Biological Molecules. NATO ASI Series, vol 139. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-6490-7_17

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  • DOI: https://doi.org/10.1007/978-94-009-6490-7_17

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-009-6492-1

  • Online ISBN: 978-94-009-6490-7

  • eBook Packages: Springer Book Archive

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