Summary
The determination of ionic organic compounds in aqueous samples by reversed-phase liquid chromatography poses problems not encountered with more non-polar organic compounds eg polynuclear aromatic hydrocarbons. The ionisation of sulphonated organic compounds must be suppressed either by the use of an ion-pairing reagent, by rendering the eluted solvent acidic, or by the use of more polar reversed-phase packings. The techniques employed for ionic suppression can be a problem, particularly when an insitu preconcentration of the organic compound is required before HPLC separation.
These problems are discussed and compromise solutions are described which vary depending upon the types of organic compound to be separated. It is recommended that paired-ion reagents are used only when other techniques fail. Satisfactory separations have been achieved using a styrene-divinylbenzene copolymer reversed-phase medium in the absence of either paired-ion reagents or buffer solutions.
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© 1984 ECSC, EEC, EAEC, Brussels and Luxembourg
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Connor, K.J., Waggott, A. (1984). Analysis of Sulphonic Acids and Other Ionic Organic Compounds Using Reversed-Phase HPLC. In: Angeletti, G., Bjørseth, A. (eds) Analysis of Organic Micropollutants in Water. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-6345-0_17
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DOI: https://doi.org/10.1007/978-94-009-6345-0_17
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