Abstract
The extraction and identification of 26 esterase isozyme bands from newly expanded leaves of Vicia faba is described. Eight of these bands were shown to be controlled by three genes: one (designated A) with two alleles and two (B & C) each with three alleles. An experiment is reported showing the use of these isozyme gene to estimate outcrossing frequencies in bean populations enclosed in pollination cages. The use of isozyme techniques in other aspects of the population genetics of Vicia faba is discussed.
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References
Bassiri, A. and Rouhani, I. (1977). Identification of broad bean cultivars based on isoenzyme patterns. Euphytica 26: 279 – 286.
Brewbaker, J. L., Upadhaya, M. D., Makinen, Y. and MacDonald, T. (1968). Isoenzyme polymorphism in flowering plants. III Gel electrophoresis methods. Physiol. Plant. 21: 930 – 940.
Brown, A. H. D. and Allard, R. W. (1970). Estimation of the mating system in open-pollinated maize populations using isozyme polymorphisms. Genetics 66: 133 – 145.
Fyfe, J. L. and Bailey, N. T. (1951). Plant breeding studies in leguminous forage crops. I. Natural cross-breeding in winter beans. J. agric. Sci., Cambs. 41: 371 – 378.
Gates, P. and Boulter, D. (1979). The use of seed isoenzymes as an aid to the breeding of field beans (Vicia faba L.). New Phytol. 83: 783 – 791.
Gates, P. and Boulter, D. (1980). The use of pollen isoenzymes as an aid to the breeding of field beans (Vicia faba L.). New Phytol. 84: 501 – 504.
Kaser, H. R. and Steiner, A. M. (1983). Subspecific classification of Vicia faba L. by protein and isozyme patterns. FABIS 7: 19 – 20.
Ladizinsky, G. (1975). Seed protein electrophoresis of the wild and cultivated species of the section faba of Vicia. Z. Pflanzenzuchtung 83: 785 – 788.
De Mora, T., Gonzalez, J. A. and Serradilla, J. M. (1983). Preliminary studies on genetic determination of electrophoresis alloenzyme bands in Vicia faba L. FABIS 7: 11 – 12.
Stuber, C. W., Goodman, M. M. and Moll, R. H. (1982). Improvement of yield and ear number resulting from selection at allozyme loci in a maize population. Crop Science 22: 737 – 740.
Stuber, C. W., Moll, R. H., Goodman, M. M., Schaffer, H. E. and Weir, B. S. (1980). Allozyme frequency changes associated with selection for increased grain yield in maize (Zea mays L.). Genetics 95: 225 – 236.
Suso, M.-J. and Moreno, M.-T. (1982). Genetic control of electrophoretic variation for glutamate-oxaloacetate-transaminase (G.O.T.) in Vicia faba L. FABIS 5: 14
Tanksley, S. D. and Rick, C. M. (1980). Isozymic gene linkage map of the Tomato: applications in genetics and breeding. Theor. appl. Genet. 57: 161 – 170
Yamamoto, K. (1975). Estimation of genetic homogeneity by isozymes from interspecific hybrids of Vicia. I. Jpn. J. Breed. 25: 60 – 64.
Yamamoto, K. (1979). Estimation of genetic homogeneity by isozymes from interspecific hybrids of Vicia. II. Jpn. J. Breed. 29: 59 – 65.
Yamamoto, K. and Plitmann, U. (1980). Isozyme polymorphism in species of the genus Vicia (Leguminosae). Jpn. J. Genetics 55: 151 – 164.
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© 1984 ECSC, EEC, EAEC, Brussels-Luxembourg
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Peat, W.E., Adham, J.Y. (1984). The Use of Isozyme Genes as Markers in the Population Genetics of Vicia Faba L.. In: Chapman, G.P., Tarawali, S.A. (eds) Systems for Cytogenetic Analysis in Vicia Faba L.. Advances in Agricultural Biotechnology, vol 11. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-6210-1_11
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DOI: https://doi.org/10.1007/978-94-009-6210-1_11
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