Restriction Endonuclease Analysis of Aujeszky’s Disease Virus DNA
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Restriction endonuclease analysis of virus DNA provides a suitable method to differentiate field isolates of Aujeszky’s disease virus (ADV). Inmost cases the differences that have been recognized in cleavage profiles of wild-type strains involved variations in the electrophoretic mobility of certain restriction fragments. Differences with respect to the presence or absence of cleavage sites were less frequently observed. Live-virus vaccine strains of ADV demonstrated unique DNA fragment patterns which were clearly different from one another and from those of wild-type virus isolates.
Restriction endonuclease analysis appeared to be a highly effective tool for (1) the identification of live-virus vaccine and wild-type virus in field cases of AD; (2) the demonstration of structural changes within the virus DNA during attenuation of virulent virus in tissue culture; (3) the study of persistence and spread of ADV within a pig herd and to trace the origin of infection; (4) the identification of the virus(es) excreted after corticosteroid treatment of latently infected pigs.
KeywordsSodium Dodecyl Sulfate Herpes Simplex Virus Type Restriction Endonuclease Analysis Cleavage Pattern Virulent Virus
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