Restriction Endonuclease Analysis of Aujeszky’s Disease Virus DNA

  • A. L. J. Gielkens
  • J. T. van Oirschot
  • F. W. van Schie
  • B. Toma
Part of the Current Topics in Veterinary Medicine and Animal Science book series (CTVM, volume 29)


Restriction endonuclease analysis of virus DNA provides a suitable method to differentiate field isolates of Aujeszky’s disease virus (ADV). Inmost cases the differences that have been recognized in cleavage profiles of wild-type strains involved variations in the electrophoretic mobility of certain restriction fragments. Differences with respect to the presence or absence of cleavage sites were less frequently observed. Live-virus vaccine strains of ADV demonstrated unique DNA fragment patterns which were clearly different from one another and from those of wild-type virus isolates.

Restriction endonuclease analysis appeared to be a highly effective tool for (1) the identification of live-virus vaccine and wild-type virus in field cases of AD; (2) the demonstration of structural changes within the virus DNA during attenuation of virulent virus in tissue culture; (3) the study of persistence and spread of ADV within a pig herd and to trace the origin of infection; (4) the identification of the virus(es) excreted after corticosteroid treatment of latently infected pigs.


Sodium Dodecyl Sulfate Herpes Simplex Virus Type Restriction Endonuclease Analysis Cleavage Pattern Virulent Virus 
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Copyright information

© ECSC, EEC, EAEC, Brussels-Luxembourg 1984

Authors and Affiliations

  • A. L. J. Gielkens
    • 1
  • J. T. van Oirschot
    • 1
  • F. W. van Schie
    • 2
  • B. Toma
    • 3
  1. 1.Department of VirologyCentral Veterinary InstituteLelystadThe Netherlands
  2. 2.Regional Animal Health Service-GelderlandVelpThe Netherlands
  3. 3.Ecole Nationale Veterinaire d’AlfortMaisons-Alfort CedexFrance

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