Advertisement

Restriction Endonuclease Analysis of Aujeszky’s Disease Virus DNA

  • A. L. J. Gielkens
  • J. T. van Oirschot
  • F. W. van Schie
  • B. Toma
Chapter
Part of the Current Topics in Veterinary Medicine and Animal Science book series (CTVM, volume 29)

Abstract

Restriction endonuclease analysis of virus DNA provides a suitable method to differentiate field isolates of Aujeszky’s disease virus (ADV). Inmost cases the differences that have been recognized in cleavage profiles of wild-type strains involved variations in the electrophoretic mobility of certain restriction fragments. Differences with respect to the presence or absence of cleavage sites were less frequently observed. Live-virus vaccine strains of ADV demonstrated unique DNA fragment patterns which were clearly different from one another and from those of wild-type virus isolates.

Restriction endonuclease analysis appeared to be a highly effective tool for (1) the identification of live-virus vaccine and wild-type virus in field cases of AD; (2) the demonstration of structural changes within the virus DNA during attenuation of virulent virus in tissue culture; (3) the study of persistence and spread of ADV within a pig herd and to trace the origin of infection; (4) the identification of the virus(es) excreted after corticosteroid treatment of latently infected pigs.

Keywords

Sodium Dodecyl Sulfate Herpes Simplex Virus Type Restriction Endonuclease Analysis Cleavage Pattern Virulent Virus 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Bartha, A. 1961. Experimental reduction of virulence of Aujeszky’s disease virus. Magy. Allatorv. Lap., 16, 42–45.Google Scholar
  2. Bartha, A., Elak, S. and Benyeda, J. 1969. Trypsin- and heat-resistance of some strains of the herpesvirus group. Acta Vet. Hung., 19, 97–99.Google Scholar
  3. Baskerville, A., McFerran, J.B. and Dow, C. 1973. Aujeszky’s disease in pigs. Vet. Bull., 43, 465–480.Google Scholar
  4. Ben-Porat, T., Rixon, R.J. and Blankenship, M.L. 1979. Analysis of the structure of the genome of pseudorabies virus. Virology, 95, 285–294.PubMedCrossRefGoogle Scholar
  5. Berns, A.J.M., Lai, M.H.T., Bosselman, R.A., McKennett, M.A., Bacheler, L.T., Fan, H., Robanus Maandag, E.C., Van der Putten, H. and Verma, I.M. 1980. Molecular cloning of unintegrated and a portion of integrated Maloney murine leukemia viral DNA in bacteriophage lambda. J. Virol., 36, 254–263.PubMedGoogle Scholar
  6. Buchman, T.G., Roizman, B., Adams, G. and Stover, B.H. 1978. Restriction endonuclease fingerprinting of herpes simplex virus DNA: a novel epidemiological tool applied to a nosocomial outbreak. J. Inf. Dis., 138, 488–498.CrossRefGoogle Scholar
  7. Denhardt, D.T. 1966. A membrane filter technique for the detection of complementary DNA. Biochem. Biophys. Res. Commun., 23, 641–646.PubMedCrossRefGoogle Scholar
  8. Geck, P., Nagy, E. and Lomniczi, B. 1982. Differentiation between Aujeszky’s disease virus strains of different virulence by restriction enzyme analysis of the DNA. Magy. Allatorv. Lap., 37, 651–656.Google Scholar
  9. Gielkens, A.L.J, and Berns, A.J.M. 1982. Differentiation of Aujeszky’s disease virus strains by restriction endonuclease analysis of the viral DNA’s. In “Current Topics in Veterinary Medicine and Animal Science” (Ed. G. Wittmann and S.A. Hall). (The Hague, Martinus Nijhoff). Vol. 17, pp. 3–13.Google Scholar
  10. Hayward, G.S., Frenkel, H. and Roizman, B. 1975. Anatomy of herpes simplex DNA: strain differences and heterogeneity in the locations of restriction endonuclease cleavage sites. Proc. Nat. Acad. Sci. USA, 72 1768–1772.PubMedCrossRefGoogle Scholar
  11. Lonsdale, D.M., Brown, S.M., Subak-Sharpe, J.H., Warren, K.G. and Koprowski, H. 1979. The polypeptide and the DNA restriction enzyme profiles of spontaneous isolates of herpes simplex virus type 1 from explants of human trigeminal, superior cervical and vagus ganglia. J. Gen. Virol., 43, 151–171.PubMedCrossRefGoogle Scholar
  12. Ludwig, H., Heppner, B. and Herrmann, S. 1982. The genomes of different field isolates of Aujeszky’s disease. In “Current Topics in Veterinary Medicine and Animal Science” (Ed. G. Wittmann and S.A. Hall). (The Hague, Martinus Nijhoff). Vol. 17, pp.15–20.Google Scholar
  13. Van Oirschot, J.T. and Gielkens, A.L.J. In vivo and in vitro reactivation of latent pseudorabies virus in pigs born to vaccinated sows. Am. J. Vet. Res., in press.Google Scholar
  14. Van Oirschot, J.T. and Gielkens, A.L.J. Intranasal vaccination of pigs against pseudorabies. 2. Absence of vaccine virus latency and failure to prevent latency of virulent virus. Am. J. Vet. Res., in press.Google Scholar
  15. Preston, V.G., Davison, A.J., Marsden, H.S., Timbury, M.C., Subak-Sharpe, J.H. and Wilkie, N.M. 1978. Recombinants between herpes simplex virus type 1 and 2: analysis of genome structures and expression of immediate-early polypeptides. J. Virol., 28, 499–517.PubMedGoogle Scholar
  16. Quint, W., Quax, W., Van der Putten, H. and Berns, A. 1981. Characterization of AKR murine leukemia virus sequences in AKR mouse substrains and structure of integrated recombinant genomes in tumor tissues. J. Virol., 39, 1–10.PubMedGoogle Scholar
  17. Ribgy, P.W.J., Dieckmann, M., Rhodes, C. and Berg, P. 1977. Labelling deoxyribonucleic acid to high specific activity in vitro by nick-translation with DNA polymerase I. J. Mol. Biol., 113, 237–251.CrossRefGoogle Scholar
  18. Rixon, F.J. and Ben-Porat, T. 1979. Structural evolution of the DNA of pseudorabies-defective viral particles. Virology, 97, 151–163.PubMedCrossRefGoogle Scholar
  19. Rubenstein, A.S. and Kaplan, A.S. 1975. Electron microscopic studies of the DNA of defective and standard pseudorabies virions. Virology, 66, 385–392.PubMedCrossRefGoogle Scholar
  20. Skoda, R., Brauner, I, Sadecky, E. and Mayer, V. 1964. Immunisation against Aujeszky’s disease with live vaccine. I. Attenuation of virus and some properties of attenuated strains. Acta Virol., Praque, 8, 1–9.Google Scholar
  21. Southern, W.M. 1975. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J. Mol. Biol., 98, 503–517.PubMedCrossRefGoogle Scholar
  22. Tatarov, G. 1968. Apathogener mutant des Aujeszky-virus, induziert von 5-Jodo-2-Deoxyuridin (JUDR). Zentbl. Vet. Med., 15B, 847–853.Google Scholar
  23. Toma, B. 1979. Obtention et caractérisation d’une souche thermosensible de virus de la maladie d’Aujeszky (Souche Alf ort 26). Ree. Med. Vet., 155, 131–137.Google Scholar
  24. Toma, B., Brun, A., Chappuis, G. and Terre, J. 1979. Propriétés biologiques d’une souche thermosensible (Alfort 26) de virus de la maladie d’Aujeszky. Ree. Med. Vet., 155, 245–252.Google Scholar
  25. Walboomers, J.M.M. and Ter Schegget, J. 1976. A new method for the isolation of herpes simplex virus type 2 DNA. Virology, 74, 256–258.PubMedCrossRefGoogle Scholar

Copyright information

© ECSC, EEC, EAEC, Brussels-Luxembourg 1984

Authors and Affiliations

  • A. L. J. Gielkens
    • 1
  • J. T. van Oirschot
    • 1
  • F. W. van Schie
    • 2
  • B. Toma
    • 3
  1. 1.Department of VirologyCentral Veterinary InstituteLelystadThe Netherlands
  2. 2.Regional Animal Health Service-GelderlandVelpThe Netherlands
  3. 3.Ecole Nationale Veterinaire d’AlfortMaisons-Alfort CedexFrance

Personalised recommendations