Detection of Viral Nucleic Acids in Cell Cultures and in Clinical Specimens by Spot Hybridization
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Nucleic acid hybridization was used for detection of adenovirus DNA directly in clinical specimens and enterovirus RNA in infected cells. DNA in 40 stool specimens, including 18 adenovirus positive and 22 adenovirus negative samples according to a highly sensitive radioimmunoassay (RIA), was spotted onto a nitrocellulose filter and hybridized with radioactively labelled adenovirus type 2 DNA probe. Sixteen specimens positive by RIA and one negative by RIA were positive in the hybridization test. A cloned partial copy of the coxsackie B3 virus genome was used to detect enteroviruses in infected cells. Cells infected with coxsackieviruses A and B, echo and polioviruses gave positive signals in the hybridization assay, whereas cells infected with other viruses did not.
KeywordsStool Specimen Hybridization Assay Nitrocellulose Filter Viral Nucleic Acid Hybridization Test
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