Capture Assays for the Detection of Virus-Specific IgM Antibody
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Class-specific antibody capture assays have been developed for a number of human virus infections, in particular for the detection of IgM antibody. These assays, MACRIA’s, have a number of advantages over the more conventional indirect solid-phase immunoassays. They are sensitive and unaffected by rheumatoid factor and particularly suited to detecting specific IgM in sera from neonates. While veterinarians will need to develop species-specific anti-μFc antisera, the compensation is that once this is done, the pair of appropriately balanced reagents, virus antigen and its labelled antibody, will be suitable to diagnose recent infection by a given virus in any animal species.
KeywordsRheumatoid Factor Capture Assay Rubella Virus Infection Single Dilution Indirect Immunoassay
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