Abstract
Class-specific antibody capture assays have been developed for a number of human virus infections, in particular for the detection of IgM antibody. These assays, MACRIA’s, have a number of advantages over the more conventional indirect solid-phase immunoassays. They are sensitive and unaffected by rheumatoid factor and particularly suited to detecting specific IgM in sera from neonates. While veterinarians will need to develop species-specific anti-μFc antisera, the compensation is that once this is done, the pair of appropriately balanced reagents, virus antigen and its labelled antibody, will be suitable to diagnose recent infection by a given virus in any animal species.
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References
Cohen, B.J., Mortimer, P.P. and Pereira, M.S. 1983. Diagnostic assays with monoclonal antibodies for the human serum parovirus-like virus (SPLV). J. Hygiene, 91, 113–130.
Diament, J.A. and Pepys, J. 1978. Immunosorbent separation of IgG and IgM for radioimmunoassay of specific antibodies. In “Affinity Chromatography” (Eds. Hoffman-Ostenkof et al.). (Pergamon Press, Oxford, England), pp. 229–231.
Gerlich, W.H. and Luer, W. 1979. Selective detection of IgM-antibody against core antigen of the hepatitis B virus by modified enzyme immuneassay. J. Med. Virol., 4, 227–238.
McFerran, J.B., Dane, D.S., Briggs, E.M., Connor, T. and Nelson, R. 1968. Further investigations on enterovirus-neutralising substance in human and animal sera. J. Path, and Bact., 95, 93–99.
Meurman, O.H., Wiljanen, M.K. and Granfors, K. 1977. Solid-phase radioimmunoassay of rubella IgM antibodies: comparison with sucrose gradient centrifugation test. J. Clin. Microbiol., 5, 257–262.
Morgan-Capner, P., Tedder, R.S. and Mace, J.E. 1983. Rubella-specific IgM reactivity in sera from cases of infectious mononucleosis. J. Hygiene, 90, 407–413.
Pattison, J.R. and Dane, D.S. 1975. The detection of specific IgM antibodies following infection with rubella virus. J. Clin. Pathol., 28, 377–382.
Salacinski, P., Hope, J., McLean, C., Clement-Jones, V., Sykes, J., Price, J. and Lowry. P.J. 1979. A new simple method which allows theoretical incorporation of radioiodine into proteins and peptides without damage. J. Endocrinol., 81, 131–137.
Sutherland, S. and Briggs, J.D. 1983. The detection of antibodies to cytomegalovirus in the sera of renal transplant patients by an IgM capture assay. J. Med. Virol., 11, 147–159.
Tedder, R.S. and Wilson-Croome, R. 1980. Detection by radioimmunoassay of IgM class antibody to hepatitis B core antigen: a comparison of two methods. J. Med. Virol., 6, 235–247.
Tedder, R.S., Yao, J.L. and Anderson, M.J. 1982. The production of monoclonal antibodies to rubella haemagglutinin and their use in antibody-capture assays for rubella-specific IgM. J. Hygiene, 88, 335–350.
Tedder, R.S., Guarascio, P., Yao, J.L., Lord, R.B. and Eddlestone, A.L.W.F. 1983. Production of monoclonal antibodies of hepatitis B surface and core antigens, and use in the detection of viral antigens in liver biopsies. J. Hygiene, 90, 135–142.
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© 1984 ECSC, EEC, EAEC, Brussels-Luxembourg
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Tedder, R.S. (1984). Capture Assays for the Detection of Virus-Specific IgM Antibody. In: McNulty, M.S., McFerran, J.B. (eds) Recent Advances in Virus Diagnosis. Current Topics in Veterinary Medicine and Animal Science, vol 29. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-6039-8_6
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DOI: https://doi.org/10.1007/978-94-009-6039-8_6
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