Detection of BVD Virus in Viremic Cattle by an Indirect Immunoperoxidase Technique
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An indirect peroxidase staining technique (PO) for detection of BVD-virus in the serum of infected cattle has been developed. The test, which is performed in microtitre plates is at least as sensitive as the fluorescent antibody technique (FA) and is easier to perform and read. The reagents in the PO test are a hyperimmune anti-BVD-virus serum raised in a goat by immunising with BVD virus grown in goat cells. Peroxidase-labelled immuno-purified bovine antibodies to goat IgG are used in the second step. BVD virus was demonstrated in 12 (0.9%) of 1332 normal randomly collected cattle sera from 2 slaughterhouses, 1042 (78%) of these had neutralising antibodies to BVD virus. Of 363 cattle from 13 herds that had recently experienced losses due to BVD, 317 (87%) had antibodies to BVD, but of the 46 seronegative animals 38 (83%) were found to be viremic. In animals with clinical disease, titres of BVD virus as assayed by PO staining were found to be as high as 105.5 TCID50/0.01 ml serum. The mean titre of aninials with disease was found to be 103.1 TCID50/0.01 ml and 102.6 TCID50/0.01 ml for apparently healthy animals. The PO staining technique for detection of BVD virus is a practical test for detection of viremic animals in infected herds and for diagnosing cases of BVD.
KeywordsBovine Viral Diarrhoea Virus Bovine Viral Diarrhoea Bovine Viral Diarrhoea Virus Infection Fluorescent Antibody Staining Bovine Viral Diarrhoea Virus Strain
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