Immunoelectrophoretic Techniques

  • K. Dalsgaard
Part of the Current Topics in Veterinary Medicine and Animal Science book series (CTVM, volume 29)


“Practical and theoretical aspects of immunoelectrophoretic techniques applied to veterinary virus diagnosis are discussed. Included are counter current electrophoresis, rocket electrophoresis, and crossed Immunoelectrophoresis. Aspects of the latter technique comprises the production of specific antisera to individual viral antigens”.


Bovine Viral Diarrhoea Virus Specific Antiserum African Swine Fever African Swine Fever Virus Counter Current 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Axelsen, N.H., Krøll, J. and Weeke, B. 1973. A manual of quantitative immunoelectrophoresis. (Blackwell Scientific Publications, Oxford).Google Scholar
  2. Cho, H.J. and Ingram, D.G. 1972. Antigen and antibody in Aleutian Disease in mink. 1. Precipitation reaction by agar-gel electrophoresis. J. Immunol., 108, 2, 555–557.PubMedGoogle Scholar
  3. Crowle, A.J. and Cline, L. Jewell. 1977. An improved stain for immunodiffusion tests. J. Immunol. Methods, 17, 379–381.PubMedCrossRefGoogle Scholar
  4. Dalsgaard, K. 1982. Precipitating antigens involved in protection against Aujeszky1s disease after natural infection and after immunisation with inactivated vaccine. In “Current Topics in Veterinary Medicine and Animal Science. Vol. 17” (Eds. G. Wittmann and S.A. Hall) (Martinus, Nijhoff, The Hague), pp 107–115.Google Scholar
  5. Dalsgaard, K. and Overby, E. 1977. Immunity against challenge with swine fever virus induced by a virus-specified glycopeptide isolated from infected cells. In “Agricultural Research Seminar on Hog cholera and African swine fever. Hannover, 1977” (CEC publication EUR. 5904) (CEC, Luxembourg). pp 70–74.Google Scholar
  6. Dalsgaard, K., Overby, E. and Sanchez-Botija, C. 1977. Crossed immuno-electrophoretic characterization of virus-specified antigens in cells infected with African swine fever virus. J. Gen. Virol., 36, 203–206.PubMedCrossRefGoogle Scholar
  7. Dalsgaard, K., Overby, E., Metzger, J.J. and Basse, A. 1979. Rapid method for screening of immunoglobulins in porcine fetuses, using rocket immunoelectrophoresis. Acta Vet. Scand., 20, 313–320.PubMedGoogle Scholar
  8. Grabar, P. and Williams, C.A. 1953. Methode permettant 1’etude conjugee des propriétés electrophoretiques d’un melange de proteines; application au serum sanguin. Biochim. biophys. Acta, 10, 193–198.PubMedCrossRefGoogle Scholar
  9. Norrild, B. and Vestergaard, B.F. 1977. Polyacrylamide gel electrophoretic analysis of herpes simplex virus type 1 immunoprecipitates obtained by quantitative immunoelectrophoresis in antibody containing agarose gel. J. Virol., 22, 113–117.PubMedGoogle Scholar
  10. Pan, I.C., Doer, C.J. and Hess, W.R. 1972. African swine fever. Application of immunoelectroosmophoresis for the detection of the antibody. Can. J. Comp. Med., 36, 309–316.PubMedGoogle Scholar
  11. Sørensen, K.J. 1980. A serological survey for swine vesicular disease in Denmark. Acta Vet. Scand., 21, 324–329.PubMedGoogle Scholar
  12. Sørensen, K.J., Askaa, J. and Dalsgaard, K. 1980. Assay for antibody in pig fetuses infected with porcine parvovirus. Acta Vet. Scand., 21, 312–317.PubMedGoogle Scholar
  13. Terpstra, C. and van Oirschot, J. 1975. Immunoelectroosmpphoresis for detection of antibody against swine fever. Agricultural Research Seminar on diagnosis and epizootiology of classical swine fever. Amsterdam 1975. (CEC publication EUR. 5486). (CEC, Luxemburg). pp 198–199.Google Scholar

Copyright information

© ECSC, EEC, EAEC, Brussels-Luxembourg 1984

Authors and Affiliations

  • K. Dalsgaard
    • 1
  1. 1.State Veterinary Institute for Virus ResearchLindholm, KalvehaveDenmark

Personalised recommendations