Use of Monoclonal Antibodies in Virus Diagnosis

  • D. van Zaane
Part of the Current Topics in Veterinary Medicine and Animal Science book series (CTVM, volume 29)


In 1975, Köhler and Milstein discovered as a spin-off result of their fundamental studies on immunoglobulin gene-expression, that lymphocytes can be immortalized by fusion with myeloma cells and thus acquire the ability to grow indefinitely and produce unlimited amounts of highly specific antibody (Köhler and Milstein, 1975). Since then, the production and application of monoclonal antibodies (MCA) has become common practice in many research areas. The enthusiasm and excitement evoked by this discovery was recently put into words by David et al. (1981) who formulated very elegantly that “the feelings experienced by an avid science fiction enthusiast watching the first astronaut plant his foot firmly on the surface of the moon could not have been dissimilar to those experienced by an immunochemist realizing the meaning of the new application of cell fusion technology presented by Köhler and Milstein”.


Newcastle Disease Virus Bovine Viral Diarrhoea Virus Bovine Leukemia Virus Newcastle Disease Virus Strain Double Antibody Sandwich 
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Copyright information

© ECSC, EEC, EAEC, Brussels-Luxembourg 1984

Authors and Affiliations

  • D. van Zaane
    • 1
  1. 1.Virology DepartmentCentral Veterinary InstituteAJ LelystadThe Netherlands

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