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Examination of Rotavirus from Bovine and Porcine Species by Western Blotting

  • Anette G. Boetner
  • Jon Askaa
Chapter
  • 57 Downloads
Part of the Current Topics in Veterinary Medicine and Animal Science book series (CTVM, volume 29)

Abstract

The “Western blotting technique” has been applied to rotavirus isolates from bovine and porcine species. Five to six polypeptides were identified for both bovine and porcine rotavirus with estimated molecular weights (MW) of 150-160K, 140-145K, 99K, 60-65K, 40-49K and 30K, of which the 40-49K polypeptide band was the strongest. These results were obtained when the virus samples were dissociated by immersing in boiling water for 3 mins before SDS-PAGE. When the virus samples were heated for 10 mins at 56°C instead, a total dissociation of the virus proteins did not occur and only two polypeptides with estimated MW of 80-85K and 95-100K appeared, of which the 80-85K polypeptide was the most intense. The possibility that the 80-85K polypeptide consists partly of the 40-49K polypeptide is discussed.

Keywords

Western Blotting Technique Virus Sample Nitrocellulose Sheet Total Dissociation Porcine Species 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© ECSC, EEC, EAEC, Brussels-Luxembourg 1984

Authors and Affiliations

  • Anette G. Boetner
    • 1
  • Jon Askaa
    • 1
  1. 1.Department of Veterinary Virology and ImmunologyThe Royal Veterinary and Agricultural University of CopenhagenCopenhagen VDenmark

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