Examination of Rotavirus from Bovine and Porcine Species by Western Blotting
- 57 Downloads
The “Western blotting technique” has been applied to rotavirus isolates from bovine and porcine species. Five to six polypeptides were identified for both bovine and porcine rotavirus with estimated molecular weights (MW) of 150-160K, 140-145K, 99K, 60-65K, 40-49K and 30K, of which the 40-49K polypeptide band was the strongest. These results were obtained when the virus samples were dissociated by immersing in boiling water for 3 mins before SDS-PAGE. When the virus samples were heated for 10 mins at 56°C instead, a total dissociation of the virus proteins did not occur and only two polypeptides with estimated MW of 80-85K and 95-100K appeared, of which the 80-85K polypeptide was the most intense. The possibility that the 80-85K polypeptide consists partly of the 40-49K polypeptide is discussed.
KeywordsWestern Blotting Technique Virus Sample Nitrocellulose Sheet Total Dissociation Porcine Species
Unable to display preview. Download preview PDF.
- Askaa, J. 1982. Detection of antibody against porcine rotavirus in colostrum and milk by a blocking ELISA test. In Current Topics in Veterinary Medicine and Animal Science, Vol. 22. “The ELISA: Enzyme-Linked Immunosorbent Assay in Veterinary Research and Diagnosis” (Eds. R.C Wardley and J.R. Crowther). (Martinus Nijhoff, The Hague), pp. 256–266.Google Scholar
- Grauballe, P.C., Vestergaard, B.F., Meyling, A. and Genner, J. 1981. Optimized enzyme-linked immunosorbent assay for the detection of human and bovine rotavirus in stools: Comparison with electronmicroscopy, immunoelectro-osmophoresis and fluorescent antibody technique. J. Med. Virol., 7, 29–40.PubMedCrossRefGoogle Scholar
- Woode, G.N., Bridger, J.C., Jones, J.M., Flewett, T.H., Bryden, A.S., Davies, H.A. and White, G.B.B. 1976. Morphological and antigenic relationships between viruses (rotaviruses) from acute gastroenteritis of children, calves, piglets, mice and foals. Infect. Immun., 14, 804–810.PubMedGoogle Scholar