Protein Blotting: The Basic Method and its Role in Viral Diagnosis
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Protein blotting involves the transfer of a complex mixture of antigens which have been resolved in a polyacrylamide gel on to a nitrocellulose membrane where the various antigenic components are accessible to analysis with antibody. The technique can be used to detect viral antigens or to dissect the humoural response to pathogens. In this paper the basic technique is described and its uses and pitfalls are discussed.
KeywordsViral Antigen Chlamydia Trachomatis Mouse Mammary Tumour Virus Electrophoretic Transfer Infected Sheep
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