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Protein Blotting: The Basic Method and its Role in Viral Diagnosis

  • A. J. Herring
  • J. M. Sharp
Chapter
Part of the Current Topics in Veterinary Medicine and Animal Science book series (CTVM, volume 29)

Abstract

Protein blotting involves the transfer of a complex mixture of antigens which have been resolved in a polyacrylamide gel on to a nitrocellulose membrane where the various antigenic components are accessible to analysis with antibody. The technique can be used to detect viral antigens or to dissect the humoural response to pathogens. In this paper the basic technique is described and its uses and pitfalls are discussed.

Keywords

Viral Antigen Chlamydia Trachomatis Mouse Mammary Tumour Virus Electrophoretic Transfer Infected Sheep 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© ECSC, EEC, EAEC, Brussels-Luxembourg 1984

Authors and Affiliations

  • A. J. Herring
    • 1
  • J. M. Sharp
    • 1
  1. 1.Moredun Research InstituteEdinburghScotland

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