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Expression of Two Murine Gene Families in Transformed Cells and Embryogenesis

  • Conference paper
Book cover Interrelationship Among Aging, Cancer and Differentiation

Part of the book series: The Jerusalem Symposia on Quantum Chemistry and Biochemistry ((JSQC,volume 18))

Abstract

Mouse clones IAP81 and mR2 have been shown to contain different repetitive DNA elements. These clones were used to investigate the expression of their respective repetitive DNA families in myelomas, embryonal carcinoma cells (EC) and, in one case, pre-implantation embryos. IAP genes which code for intracisternal A-type viral particles are highly transcribed in myeloma and, to a lesser extent, in EC cells. While IAP transcripts of three sizes (7.2 kb, 5.3 kb and 3.8 kb) were found in myelomas, only two of the IAP RNA species (7.2 kb and 5.3 kb) were detected in EC cells. Very little IAP RNA could be detected in EC cells following differentiation. Abundant IAP RNA was visualized in 1, 2 and 4–8 cell stage embryos by in situ hybridization. The amount of IAP RNA decreases drastically in embryos at the morula stage. In similar studies using the mR2 clone, a 1.4 kb transcript was detected in myeloma and EC cells. This transcript is not detected in differentiated EC cells or liver and brain of adult mice.

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Author information

Authors and Affiliations

  1. Department of Biology, The Johns Hopkins University, Baltimore, Maryland, 21218, USA

    Jeffrey A. Moshier, Richard A. Morgan & Ru Chih C. Huang

Authors
  1. Jeffrey A. Moshier
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  2. Richard A. Morgan
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  3. Ru Chih C. Huang
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Editor information

Editors and Affiliations

  1. Institut de Biologie Physico-Chimique (Fondation Edmond de Rothschild), Paris, France

    Bernard Pullman

  2. Division of Biophysics, School of Hygiene and Public Health, The Johns Hopkins University, Baltimore, Maryland, USA

    Paul O. P. Ts’o

  3. National Institute on Aging, National Institutes of Health, Bethesda, Maryland, USA

    Edmond L. Schneider

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© 1985 D. Reidel Publishing Company, Dordrecht, Holland

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Moshier, J.A., Morgan, R.A., Huang, R.C.C. (1985). Expression of Two Murine Gene Families in Transformed Cells and Embryogenesis. In: Pullman, B., Ts’o, P.O.P., Schneider, E.L. (eds) Interrelationship Among Aging, Cancer and Differentiation. The Jerusalem Symposia on Quantum Chemistry and Biochemistry, vol 18. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-5466-3_11

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  • DOI: https://doi.org/10.1007/978-94-009-5466-3_11

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-010-8913-5

  • Online ISBN: 978-94-009-5466-3

  • eBook Packages: Springer Book Archive

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