Abstract
In this review we show that Electron Spin Resonance (ESR) can be used to investigate the time scale and specificity of lipid-lipid and lipid—protein contacts in membranes. Details are given of the simulation of the spectra obtained with labeled lipids either covalently attached to the boundary of rhodopsin or freely diffusing in rhodopsin containing vesicles. It is shown that the time scale of conventional ESR is idealy suited to the determination of the collision frequencies between lipids and proteins and/or lipids-lipids in membranes. In addition we show that the use of a mixture of 14N and 15N nitroxides allows one to detect unambiguously the collision frequency between unlike molecules such as two different phospholipids or a phospholipid and a labeled protein.
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References
Devaux, P. F. (1983) in “Biological Magnetic Resonance” vol. 5, Berliner, L.J. and Reuben, J., eds (Plenum Press, New York), pp. 183–299.
Devaux, P. F. and Seigneuret, M. (1984) Biochim. Biophys. Acta, in press.
Berliner, L.J., ed. (1976) Spin Labeling — Theory and Applications Vol. 1 (Academic Press, New York).
Berliner, L.J., ed. (1979) Spin Labeling — Theory and Applications Vol. 2 (Academic Press, New York).
Marsh, D. (1981) in “Membrane Spectroscopy”, Grell, E., ed. (Springer Verlag, Berlin) pp. 51–142.
Gaffney, B.J. and Mc Connell, H. M. (1974) J. Magn. Res. 16, 1–28.
Favre, E., Baroin, A., BienvenĂĽe, A. and Devaux, P. F. (1979) Biochemistry 18, 1156-
Davoust, J., Schoot, B. M. and Devaux, P.F. (1979) Proc. Natl. Acad. Sci. USA 76, 2755–2759.
Lee, A.G., XI International Conference on Magnetic Resonance in Biological Systems, Fort Aguada Beach Resort, Goa, India. sept. 1984, abstract IT 13.
Devaux, P.F., Scandella, C.J. and Mc Connell, H.M. (1973) J. Magn. Res. 9, 474–485.
Sackmann, E. and Traüble, H. (1972) J. Am. Chem. Soc. 94, 4492–4498.
Seigneuret, M., Davoust, J., Hervé, P. and Devaux, P.F. (1981) Biochimie 63, 867–870.
Davoust, J., Seigneuret, M., Hervé, P. and Devaux, P.F. (1983) Biochemistry 22, 3137–3145.
Davoust, J., Seigneuret, M., Hervé, P. and Devaux, P.F. (1983) Biochemistry 22, 3145–3151.
Popp, C. and Hyde, J.S. (1982) Proc. Natl. Acad. Sci. USA 79, 2559–2563.
Feix, J.R., Popp, C., Venkataramu, S.D., Beth, A. H., Park, J. H. and Hyde, J.S. (1984) Biochemistry 23, 2293–2299.
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Seigneuret, M., Davoust, J., Devaux, P.F. (1985). Simulation of the Electron Spin Resonance Spectra of Spin-Labeled Fatty Acids in Protein-Containing Model Membranes. In: Daudel, R., Korb, JP., Lemaistre, JP., Maruani, J. (eds) Structure and Dynamics of Molecular Systems. Structure and Dynamics of Molecular Systems, vol 1. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-5351-2_11
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DOI: https://doi.org/10.1007/978-94-009-5351-2_11
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